BMI-1 Antibody (JJ093-3)

Western Blot: BMI-1 Antibody (JJ093-3) [NBP2-67916] - Western blot analysis of BMI-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: PC-12 cell lysate Lane 3: SW480 cell lysate

Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (JJ093-3) [NBP2-67916] - Staining Bmi1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: BMI-1 Antibody (JJ093-3) [NBP2-67916] - Analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: PC-12

Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (JJ093-3) [NBP2-67916] - Staining Bmi1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (JJ093-3) [NBP2-67916] - Staining Bmi1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: BMI-1 Antibody (JJ093-3) [NBP2-67916] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: BMI-1 Antibody (JJ093-3) [NBP2-67916] - Analysis of paraffin-embedded human lung cancer tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: BMI-1 Antibody (JJ093-3) [NBP2-67916] - Analysis of paraffin-embedded human tonsil tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: BMI-1 Antibody (JJ093-3) [NBP2-67916] - Analysis of paraffin-embedded rat smooth muscle tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.

Product Discontinued

BMI-1 Antibody (JJ093-3) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human BMI-1 aa 282-326 / 326. (SwissProt: P35226 Human; Entrez Gene:307151 Rat)
• Localization: Nucleus, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BMI1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for BMI-1 Antibody (JJ093-3)

• B lymphoma Mo-MLV insertion region 1 homolog (mouse)
• B lymphoma Mo-MLV insertion region 1 homolog
• BMI1 polycomb ring finger oncogene
• BMI1
• BMI-1
• FLVI2/BMI1
• MGC12685
• murine leukemia viral (bmi-1) oncogene homolog
• PCGF4
• polycomb complex protein BMI-1
• polycomb group protein Bmi1
• polycomb group ring finger 4
• RING finger protein 51
• RNF51
• RNF51Polycomb group RING finger protein 4

Background

Bmi1 polycomb ring finger oncogene regulates the cell cycle inhibitor genes p16 and p19, with implications in hematopoiesis, skeletal patterning, neurological functions, and development of the cerebellum. Bmi 1 has been shown to induce telomerase activity and immortalize human mammary epithelial cells, and extend the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. Bmi-1 is also required for efficient self-renewing cell division in adult hematopoietic and neural stem cells.

Aberrant expression of Bmi-1 has been found in several types of cancer in the bladder, skin, blood, prostate, breast, ovaries, andcolon. Its overexpression is especially pronounced in mantle cell lymphomas. Thereforem Bmi1 antibodies will be useful tools for many cancer development studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BMI-1 Antibody (NBP2-67916)(1)

Publication using NBP2-67916

• Bergin, CJ;Benoit, YD; Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity STAR protocols [PMID: 35265864] (Human)

Bioinformatics

• Gene Symbol: BMI1