Recombinant Human BMAL1 GST (N-Term) Protein

SDS-Page: Recombinant Human BMAL1 Protein [H00000406-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

• Reactivity: Hu
• Applications: WB, ELISA, MA, AP

Recombinant Human BMAL1 GST (N-Term) Protein Summary

• Description: Recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-625 of Human ARNTL
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MADQRMDISSTISDFMSPGPTDLLSSSLGTSGVDCNRKRKGSSTDYQESMDTDKDDPHGRLEYTEHQGRIKNAREAHSQIEKRRRDKMNSFIDELASLVPTCNAMSRKLDKLTVLRMAVQHMKTLRGATNPYTEANYKPTFLSDDELKHLILRAADGFLFVVGCDRGKILFVSESVFKILNYSQNDLIGQSLFDYLHPKDIAKVKEQLSSSDTAPRERLIDAKTGLPVKTDITPGPSRLCSGARRSFFCRMKCNRPSVKVEDKDFPSTCSKKKDRKSFCTIHSTGYLKSWPPTKMGLDEDNEPDNEGCNLSCLVAIGRLHSHVVPQPVNGEIRVKSMEYVSRHAIDGKFVFVDQRATAILAYLPQELLGTSCYEYFHQDDIGHLAECHRQVLQTREKITTNCYKFKIKDGSFITLRSRWFSFMNPWTKEVEYIVSTNTVVLANVLEGGDPTFPQLTASPHSMDSMLPSGEGGPKRTHPTVPGIPGGTRAGAGKIGRMIAEEIMEIHRIRGSSPSSCGSSPLNITSTPPPDASSPGGKKILNGGTPDIPSSGLLSGQAQENPGYPYSDSSSILGENPHIGIDMIDNDQGSSSPSNDEAAMAVIMSLLEADAGLGGPVDFSDLPWPL

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: BMAL1
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
• Theoretical MW: 94.49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human BMAL1 GST (N-Term) Protein

• ARNTL
• aryl hydrocarbon receptor nuclear translocator-like protein 1
• aryl hydrocarbon receptor nuclear translocator-like
• basic-helix-loop-helix-PAS orphan MOP3
• BHLHE5
• bHLHe5brain and muscle
• bHLH-PAS protein JAP3
• BMAL1
• BMAL1c
• BMAL1TIC
• Brain and muscle ARNT-like 1
• Class E basic helix-loop-helix protein 5
• JAP3
• Member of PAS protein 3
• member of PAS superfamily 3
• MOP3
• MOP3BMAL1c
• PAS domain-containing protein 3
• PASD3
• PASD3MGC47515
• TIC

Background

ARNTL( AAH41129, 1 a.a. - 626 a.a.) recombinant protein with GST.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Publications for BMAL1 Recombinant Protein (H00000406-P01)(29)

Publications using H00000406-P01

• Chen Y, Cui T, Xiao S et al. Hepatic ZBTB22-mediated Detoxification Ameliorates Acetaminophen-induced Liver Injury by Inhibiting Pregnane X Receptor Signaling iScience 2023-03-01 [PMID: 36950116]
• Gonzalez-Teran B, Pittman M, Felix F et al. Integration of Protein Interactome Networks With Congenital Heart Disease Variants Reveals Candidate Disease Genes SSRN Electronic Journal 2020-12-08
• Horvath C, Houriet J, Moser C et al. Genkwanin Glycosides from the Phaleria Nisidai Extract Improve Glucose Homeostasis by Stimulating Insulin-Independent Glucose Uptake SSRN Electronic Journal 2022-01-04
• Casagrande S, Dzialo M, Trost L et al. Mitochondrial metabolism in blood more reliably predicts whole-animal energy needs compared to other tissues iScience 2023-10-01 [PMID: 38025793]
• Qu Y, He Y, Wang Y et al. Targeted down-regulation of SRSF1 exerts anti-cancer activity in OSCC through impairing lysosomal function and autophagy iScience 2023-10-01 [PMID: 38025785]
• Xue H, Xu X, Yan Z et al. Genome-wide association study of hippocampal BOLD-CBF correlation in Chinese Han population iScience 2023-09-01 [PMID: 37822511]
• Chen Y, Sadiq S, Tian J Et al. RNA Virome Composition Is Shaped by Sampling Ecotype SSRN Electronic Journal 2021-10-10
• Barone I, Gilette NM, Hawks-Mayer H et al. Synaptic BMAL1 phosphorylation controls circadian hippocampal plasticity Science advances 2023-10-27 [PMID: 37878694] (KA)
  
  Details:
  In Vitro Kinase Assay
• Voss J, Cr�semann M, Bartling C et al. Structure-affinity and structure-residence time relationships of macrocyclic G?q protein inhibitors iScience 2023-03-01 [PMID: 37091255]
• Fehr T, Janssen W, Park J et al. Neonatal exposures to sevoflurane in rhesus monkeys alter synaptic ultrastructure in later life iScience 2022-11-01 [PMID: 36567715]
• PUtz C, van den Berg B, Lorist M Dynamic modulation of neural feedback processing and attention during spatial probabilistic learning iScience 2022-04-01 [PMID: 35602968]
• Horvath, T;Ihekweazu, F;Haidacher, S;Ruan, W;Engevik, K;Fultz, R;Hoch, K;Luna, R;Oezguen, N;Spinler, J;Haag, A;Versalovic, J;Engevik, M; Bacteroides ovatus colonization influences the abundance of intestinal short chain fatty acids and neurotransmitters iScience [PMID: 35494230]
• Feng H, Zhang X, Zhang Y et al. Design of an omnidirectional camouflage device with anisotropic confocal elliptical geometry in thermal-electric field iScience 2022-04-01 [PMID: 35479400]
• Bing L, Di L, Haibo N et al. The circadian clock regulator Bmal1 affects traumatic brain injury in rats through the p38 MAPK signalling pathway. Brain Res Bull. 2021-11-11 [PMID: 34774994]
• Sun Y, Zeng Y, Zhang T Quantum superposition inspired spiking neural network iScience 2021-08-01 [PMID: 34401664]
• Dawson C, Irwin S, Backman L et al. Molecular Basis of C-S Bond Cleavage in the Glycyl Radical Enzyme Isethionate Sulfite-Lyase Cell Chem Biol 2021-03-27 [PMID: 33773110]
• Luse D, Parida M, Spector B et al. A Unified View of the Sequence and Functional Organization of the Human RNA Polymerase II Promoter Nucleic Acids Res 2020-06-30 [PMID: 32597978]
• Petkau N, Budak H, Zhou X et al. Acetylation of BMAL1 by TIP60 controls BRD4-P-TEFb recruitment to circadian promoters. Elife. 2019-07-11 [PMID: 31294688]
• Johnson B, VanBlargan LA, Xu W et al. Human IFIT3 Modulates IFIT1 RNA Binding Specificity and Protein Stability. Immunity. 2018-03-20 [PMID: 29525521] (Human)
• Kooreman NG, Kim Y, de Almeida PE et al. Autologous iPSC-Based Vaccines Elicit Anti-tumor Responses In Vivo. Cell Stem Cell 2018-04-05 [PMID: 29456158] (Human)
• Isoda T, Moore AJ, He Z et al. Non-coding Transcription Instructs Chromatin Folding and Compartmentalization to Dictate Enhancer-Promoter Communication and T Cell Fate. Cell 2017-09-21 [PMID: 28938112] (Human)
• Bohlen CJ, Bennett FC, Tucker AF et al. Diverse Requirements for Microglial Survival, Specification, and Function Revealed by Defined-Medium Cultures Neuron 2017-05-17 [PMID: 28521131] (Human)
• Severson KS, Xu D, Van de Loo M et al. Active Touch and Self-Motion Encoding by Merkel Cell-Associated Afferents Neuron 2017-05-03 [PMID: 28434802] (Human)
• Whitaker WR, Shepherd ES, Sonnenburg JL. Tunable Expression Tools Enable Single-Cell Strain Distinction in the Gut Microbiome. Cell 2017-04-20 [PMID: 28431251] (Human)
• Gasset-RoSa F, Chillon-Marinas C, Goginashvili A et al. Polyglutamine-Expanded Huntingtin Exacerbates Age-Related Disruption of Nuclear Integrity and Nucleocytoplasmic Transport. Neuron 2017-04-05 [PMID: 28384474] (Human)
• Dillon GM, Tyler WA, Omuro KC et al. CLASP2 Links Reelin to the Cytoskeleton during Neocortical Development. Neuron. 2017-03-22 [PMID: 28285824] (Human)
• Rai R, Hu C, Broton C et al. NBS1 Phosphorylation Status Dictates Repair Choice of Dysfunctional Telomeres. Mol. Cell. 2017-03-02 [PMID: 28216226] (Human)
• Chen V, Chang J, Zhang J et al. CD36 Restricts Lipid-Associated Macrophages Accumulation in White Adipose Tissues During Atherogenesis Cell Reports 2023-09-05
• Bose S, Ganguly S, Kumar S, Boockfor FR et al. A Pit-1 Binding Site Adjacent to E-box133 in the Rat PRL Promoter is Necessary for Pulsatile Gene Expression Activity. Neurochem Res 2016-02-15 [PMID: 26875730]

Bioinformatics

• Gene Symbol: BMAL1