BMAL1 Antibody

Western Blot: BMAL1 Antibody [NBP1-28802] - (0.3ug/ml) staining of Human Brain (Cerebellum) 35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

BMAL1 Antibody Summary

• Description: Novus Biologicals Goat BMAL1 Antibody (NBP1-28802) is a polyclonal antibody validated for use in WB and ELISA. Anti-BMAL1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence REKITTNCYKFKIKD corresponding to internal region according to NP_001025444.1.
• Epitope: internal region
• Specificity: This antibody is expected to recognize both reported isoforms (NP_001025444.1; NP_001025443.1). Reported variants represent identical protein (NP_001025443.1; NP_001169.3).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BMAL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: Use in ELISA reported in scientific literature (PMID:34488619). WB: Approx. 70+75 kDa band bands observed in human brain (cerebellum). lysates (calculated MW of 68.7 kDa band according to NP_001169.3 and 64.1 kDa band according to NP_001025444.1). .

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BMAL1 Antibody

• ARNTL
• aryl hydrocarbon receptor nuclear translocator-like protein 1
• aryl hydrocarbon receptor nuclear translocator-like
• basic-helix-loop-helix-PAS orphan MOP3
• BHLHE5
• bHLHe5brain and muscle
• bHLH-PAS protein JAP3
• BMAL1
• BMAL1c
• BMAL1TIC
• Brain and muscle ARNT-like 1
• Class E basic helix-loop-helix protein 5
• JAP3
• Member of PAS protein 3
• member of PAS superfamily 3
• MOP3
• MOP3BMAL1c
• PAS domain-containing protein 3
• PASD3
• PASD3MGC47515
• TIC

Background

The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating this gene and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BMAL1 Antibody (NBP1-28802)(2)

Publications using NBP1-28802

• Mishra R, Bethunaickan R, Berthier CC et al. Reversible dysregulation of renal circadian rhythm in lupus nephritis Molecular medicine (Cambridge, Mass.) 2021-09-06 [PMID: 34488619] (ELISA, Mouse)
• Fuller PM, Lu J, Saper CB. Differential rescue of light- and food-entrainable circadian rhythms. Science 2008-05-01 [PMID: 18497298]

Bioinformatics

• Gene Symbol: BMAL1
• Entrez:
  • 406(Human)
  • 11865(Mouse)
  • 29657(Rat)
• Uniprot:
  • NP_001169.3(Human)