BIVM Antibody - C-terminus - BSA Free

Western Blot: BIVM Antibody [NB600-491] - A 57 kDa band corresponding to human BIVM protein is detected using a 1:1,000 dilution of the antiserum incubated for 1 hour at room temperature. Washes with 1% Tween-20 TBS preceded reaction with HRP Gt-a-Rabbit IgG (H&L) code 611-103-122 diluted 1:10,000 and incubated for 1 hour at room temperature. The blot was developed using a chemiluminescent detection method (AP ECL 60 sec followed by a 45 sec exposure).

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BIVM Antibody - C-terminus - BSA Free Summary

• Description: This product was prepared from monospecific antiserum by delipidation and defibrination
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: BIVM peptide corresponding to a region near carboxy terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). (Uniprot: Q86UB2)
• Localization: Cytoplasmic and Nuclear
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BIVM
• Purity: Delipidation and Defibrination

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:25000
  • Western Blot 1:1000
• Application Notes: This polyclonal antibody reacts human BIVM at the C-terminal region and is useful for detection of BIVM by ELISA or western blotting. For immunoblotting a 57 kDa band corresponding to human BIVM is detected. Unstimulated HeLa cells can be used as a positive control. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

This conserved protein is present in many tissues and organisms and therefore cross-reactivity with BIVM from other sources is likely but has not been determined

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Delipidation and Defibrination

Alternate Names for BIVM Antibody - C-terminus - BSA Free

• basic immunoglobulin-like variable motif-containing protein
• basic, immunoglobulin-like variable motif containing
• FLJ20159
• FLJ44301
• FLJ44556
• MGC133326

Background

BIVM (for basic, immunoglobulin-like variable motif-containing) refers to a recently identified gene product that maps to human chromosome 13q32-q33 and is predicted to encode a 503-amino acid protein. BIVM shows ubiquitous expression in normal human tissue and the presence of a 5' CpG island suggests it is a housekeeping gene. BIVM is likely essential for some aspect of basic cellular function. BIVM is highly charged and localizes to the cytoplasm and nucleus where it may bind to either DNA or RNA or associate with other cellular proteins. Significant sequence homology exists with many organisms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BIVM
• Entrez:
  • 54841(Human)
• Uniprot:
  • Q86UB2(Human)