50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Useful in Western Blot and ELISA. Use in immunprecipitation reported in scientific literature (PMID 24451375) This protein has not been tested for any functionality. This product may contain endotoxins and is not suitable for use with live cells.
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for beta-TrCP1/BTRC Recombinant Protein
beta-transducin repeat containing
F-box and WD repeats protein beta-TrCP
F-box/WD repeat-containing protein 1A
pIkappaBalpha-E3 receptor subunit
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.