Bcl-2 related protein A1 Antibody

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Western Blot: Bcl-2 related protein A1 Antibody [NBP1-45327] - Analysis of A1 expression with Jurkat cell lysate (Lane 1), mouse small intestine tissue lysate (Lane 2), and rat kidney tissue lysate (Lane 3).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Bcl-2 related protein A1 Antibody Summary

Immunogen
Synthetic peptide surrounding amino acid 37 of human A1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCL2A1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Western blotting (0.5-4 ug/ml), immunoprecipitation, and Immunohistochemistry. However, the optimal conditions should be determined individually. The A1 antibody detects A1 (Bfl-1) of human, mouse, and rat origins.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2), 0.5% BSA and 30% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for Bcl-2 related protein A1 Antibody

  • ACC-1
  • ACC-2
  • Bcl-2 related protein A1
  • BCL2A1
  • Bcl2L5
  • Bcl2-L-5
  • BCL2L5HBPA1
  • Bcl-2-like protein 5
  • BCL2-related protein A1
  • Bfl-1
  • BFL1bcl-2-related protein A1
  • GRS
  • GRSbcl2-L-5
  • hematopoietic BCL2-related protein A1
  • Hemopoietic-specific early response protein
  • Protein BFL-1
  • Protein GRS

Background

Bcl-2 related protein A1; also known as Bcl2A1 and Bfl1, is an anti-apoptotic Bcl-2 family member preferentially expressed in hematopoietic and endothelial cells (1). Bfl1 is involved in a variety of cellular activities such as embryonic development, homeostatis and tumorigenesis. Expression of Bfl1 is stimulated by NF-kB in response to inflammatory stimuli and is up-regulated by TNF-alpha, IL1-beta, IGF1, GMC-SF, CD40 and phorbol ester (2-3). Blf1 has also been shown to reduce the release of pro-apoptotic cytochrome c from the mitochondria, block caspase activation and suppress apoptosis induced by p53 (4-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol BCL2A1
Entrez
Uniprot