B4GALT4 Antibody (OTI8B6) [Janelia Fluor® 669]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI8B6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 669

B4GALT4 Antibody (OTI8B6) [Janelia Fluor® 669] Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 40-322 of human B4GALT4(NP_003769) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: B4GALT4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for B4GALT4 Antibody (OTI8B6) [Janelia Fluor® 669]

• B4Gal-T4
• beta-1,4-galactosyltransferase 4
• Beta-1,4-GalTase 4
• beta4Gal-T4
• beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase 4
• EC 2.4.1.-
• UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 4
• UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4
• UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4
• UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4

Background

B4GALT4 is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene appears to mainly play a role in glycolipid biosynthesis. Two alternatively spliced transcript variants have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: B4GALT4