Recombinant Human AUH Protein

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SDS-Page: Recombinant Human AUH Protein [NBP1-50922] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human AUH Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 68-339 of Human AUH

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSSEMKTEDE LRVRHLEEEN RGIVVLGINR AYGKNSLSKN LIKMLSKAVD ALKSDKKVRT IIIRSEVPGI FCAGADLKER AKMSSSEVGP FVSKIRAVIN DIANLPVPTI AAIDGLALGG GLELALACDI RVAASSAKMG LVETKLAIIP GGGGTQRLPR AIGMSLAKEL IFSARVLDGK EAKAVGLISH VLEQNQEGDA AYRKALDLAR EFLPQGPVAM RVAKLAINQG MEVDLVTGLA IEEACYAQTI PTKDRLEGLL AFKEKRPPRY KGE

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
AUH
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
31.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human AUH Protein

  • 3-methylglutaconyl-CoA hydratase
  • AU RNA binding protein/enoyl-CoA hydratase
  • AU RNA binding protein/enoyl-Coenzyme A hydratase
  • AU RNA-binding protein/enoyl-Coenzyme A hydratase
  • AU-binding protein/enoyl-CoA hydratase
  • AU-specific RNA-binding enoyl-CoA hydratase
  • EC 4.2.1.18
  • methylglutaconyl-CoA hydratase, mitochondrial

Background

AUH, also known as mitochondrial methylglutaconyl-CoA hydratase, is involved in the amino acid degradation pathway by catalyzing the conversion of 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA and water. It has been found to have very low enoyl-CoA hydratase activity. Recombinant human AUH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol AUH