ATG101 Overexpression Lysate (Native)

Images

 
Western Blot: C12orf44 Overexpression Lysate (Adult Normal) [NBP2-08327] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for C12orf44.

Product Details

Summary
Applications WB

Order Details

ATG101 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_001092143. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ATG101 Overexpression Lysate (Native), mRNA.
Gene
C12ORF44

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
24.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ATG101

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ATG101 Overexpression Lysate (Native)

  • ATG101
  • Atg13-interacting protein
  • autophagy-related protein 101
  • C12orf44
  • chromosome 12 open reading frame 44
  • FLJ11773

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-24738
Species: Hu, Mu, Pm
Applications: WB, IHC-P
NB100-77279
Species: Hu, Mu
Applications: WB, IP
NBP2-52419
Species: Hu
Applications: WB, ELISA, Flow, IHC, CyTOF-ready, ICC, IF
NBP1-33136
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-43644
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
AF2850
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC
NBP1-89865
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-22127
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-87487
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88883
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-71771
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-240
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IP, PLA
NBP1-87232
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF847
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NBP1-55202
Species: Hu
Applications: WB, IHC, IHC-P

Publications for ATG101 Lysate (NBP2-08327) (0)

There are no publications for ATG101 Lysate (NBP2-08327).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ATG101 Lysate (NBP2-08327) (0)

There are no reviews for ATG101 Lysate (NBP2-08327). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ATG101 Lysate (NBP2-08327) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ATG101 Products

Bioinformatics Tool for ATG101 Lysate (NBP2-08327)

Discover related pathways, diseases and genes to ATG101 Lysate (NBP2-08327). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Pathways for ATG101 Lysate (NBP2-08327)

View related products by pathway.

PTMs for ATG101 Lysate (NBP2-08327)

Learn more about PTMs related to ATG101 Lysate (NBP2-08327).

Blogs on ATG101

There are no specific blogs for ATG101, but you can read our latest blog posts.

Customers Who Bought This Also Bought

ATG101 Antibody
NBP1-88877
ATG101 Antibody
NBP1-88877

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ATG101 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol C12ORF44