ATF6 Antibody (2358C)

Immunohistochemistry: ATF6 Antibody (2358C) [NBP2-76329] - ATF6 was detected in immersion fixed paraffin-embedded sections of human prostate using Rabbit Anti-Human/Mouse/Rat ATF6 Monoclonal Antibody (Catalog # NBP2-76329) at 0.3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM) HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei.

Western Blot: ATF6 Antibody [NBP2-76329] - Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, A20 mouse B cell lymphoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.05 ug/mL of Rabbit Anti-Human/Mouse/Rat ATF6 Monoclonal Antibody (Catalog # NBP2-76329) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ATF6 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP2-76329] - ATF6 was detected in immersion fixed A549 human lung carcinoma cell line using Rabbit Anti-Human/Mouse/Rat ATF6 Monoclonal Antibody (Catalog # NBP2-76329) at 8 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei.

Flow Cytometry: ATF6 Antibody [NBP2-76329] - MCF-7 human breast cancer cell line was stained with Rabbit Anti-Human/Mouse/Rat ATF6 Monoclonal Antibody (Catalog # NBP2-76329, filled histogram) or Rabbit IgG control antibody (Catalog # MAB1050, open histogram) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Product Discontinued

ATF6 Antibody (2358C) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Partial recombinant human ATF6 protein (amino acids 1-192) [UniProt P18850]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ATF6
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular) 0.25 ug/million cells
  • Flow Cytometry 0.25 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 5 - 15 ug/ml
  • Immunohistochemistry 0.3 - 2 ug/ml
  • Immunohistochemistry-Paraffin 0.3 - 2 ug/ml
  • Western Blot 0.05 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for ATF6 Antibody (2358C)

• ACHM7
• Activating transcription factor 6 alpha
• activating transcription factor 6
• atf6 a
• ATF6 alpha
• ATF6
• ATF6A
• ATF6-alpha
• cAMP-dependent transcription factor ATF-6 alpha
• cyclic AMP-dependent transcription factor ATF-6 alpha

Background

ATF6 is a constitutively expressed, endoplasmic reticulum (ER) membrane-anchored transcription factor. ATF6 is a key transcriptional activator of the unfolded protein response (UPR), which allows mammalian cells to maintain cellular homeostasis when they are subjected to environmental and physiological stresses that target the ER (reviewed in Shen, 2005 & Prywes, 2005). The C-terminus of ATF6 is located in the ER lumen and its N-terminal DNA binding domain faces the cytosol. AFT6 plays a key role in the ER stress response by transmitting the ER stress signal across the ER membrane into the nucleus. The induction of new gene expression by ATF6 is an important aspect of the ER stress response. In response to certain stress conditions, ATF6 translocates from the ER to the Golgi. The 90 kDa full-length ATF6 is processed within the Golgi to its active 50 kDa form through sequential cleavage by site-1 and site-2 proteases (S1P and S2P). Proteolytic activation of ATF6 in the ER stress response is a mechanism to regulate membrane-bound factors, and is referred to as regulated intramembrane proteolysis. The N-terminal active ATF6 translocates to the nucleus where it binds to ER stress-response elements in ER stress-response genes (ERSRGs). ATF6 is a potent transcriptional activator of ERSRGs. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATF6 Antibody (NBP2-76329)(2)

Publications using NBP2-76329

• Kulesskaya N, Bhattacharjee A, Holmström KM et al. HER-096 is a CDNF-derived brain-penetrating peptidomimetic that protects dopaminergic neurons in a mouse synucleinopathy model of Parkinson's disease Cell chemical biology 2023-11-24 [PMID: 38039968]
• SOZEN E, DEMIREL-YALCINER T, DAMLA DEMIR D Et al. SREBP1c silencing reduces endoplasmic reticulum stress and related apoptosis in oleic acid induced lipid accumulation Marmara Medical Journal 2021-10-31 (ICC/IF, Mouse)

FAQs for ATF6 Antibody (NBP2-76329). (Showing 1 - 1 of 1 FAQs).

1. We are looking for an antibody specific to ATF6 alpha that will not cross-react with ATF6 beta. Would you please help confirm if any of the following are ATF6 alpha specific antibodies: NBP1-40256, NBP1-77251, NBP1-76675 & NBP1-41439?
   • The antibodies NBP1-77251 and NBP1-76675 are specific to ATF6 alpha and should not cross-react with ATF6 beta.

Bioinformatics

• Gene Symbol: ATF6