Human Aspartate Aminotransferase ELISA Kit (Colorimetric)

ELISA: Human Aspartate Aminotransferase ELISA Kit (Colorimetric) [NBP2-69876] - Samples were spiked with high concentrations of Human Aspartate Aminotransferase and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Aspartate Aminotransferase ELISA Kit (Colorimetric) [NBP2-69876] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Human Aspartate Aminotransferase ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human AST in samples. No significant cross-reactivity or interference between Human AST and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.58%
• Intra-Assay: CV% < 5.55%
• Spike Recovery: 89-103%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: GOT1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Aspartate Aminotransferase ELISA Kit (Colorimetric)

• aspartate aminotransferase, cytoplasmic
• AST1
• ASTQTL1
• CASPAT
• CCAT
• Cysteine aminotransferase, cytoplasmic
• Cysteine transaminase, cytoplasmic
• EC 2.6.1.1
• EC 2.6.1.3
• GIG18
• Glutamate oxaloacetate transaminase 1
• glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
• GOT1
• growth-inhibiting protein 18
• SGOT
• Transaminase A

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Aspartate Aminotransferase ELISA Kit (NBP2-69876). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: GOT1