Aspartate Aminotransferase Antibody

Western Blot: Aspartate Aminotransferase Antibody [NB100-93403] - Staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NB100-93403] - Staining of human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NB100-93403] - Staining of human heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Aspartate Aminotransferase Antibody Summary

• Description: Novus Biologicals Goat Aspartate Aminotransferase Antibody (NB100-93403) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Aspartate Aminotransferase Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RSYRYWDAEKR corresponding to internal region according to NP_002070.1.
• Epitope: C-RSYRYWDAEKR
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GOT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 5 ug/ml
  • Immunohistochemistry-Paraffin 2 - 5 ug/ml
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.01 - 1 ug/ml
• Application Notes: WB: Approx 40 kDa band observed in human brain (cerebellum), Liver and skeletal muscle lysates (calculated MW of 46.2 kDa according to NP_002070.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (PMID: 2185471). IHC: Human heart shows strong staining of the capillary network between the myocardial fibres in transverse secion.
• Control:

  Aspartate Aminotransferase Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Aspartate Aminotransferase Antibody

• aspartate aminotransferase, cytoplasmic
• AST1
• ASTQTL1
• CASPAT
• CCAT
• Cysteine aminotransferase, cytoplasmic
• Cysteine transaminase, cytoplasmic
• EC 2.6.1.1
• EC 2.6.1.3
• GIG18
• Glutamate oxaloacetate transaminase 1
• glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
• GOT1
• growth-inhibiting protein 18
• SGOT
• Transaminase A

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aspartate Aminotransferase Antibody (NB100-93403)(1)

Publication using NB100-93403

• Teichberg VI. From the liver to the brain across the blood-brain barrier. Proc Natl Acad Sci U S A 2007-05-01 [PMID: 17463093]

Bioinformatics

• Gene Symbol: GOT1
• Entrez:
  • 2805(Human)
  • 24401(Rat)
• Uniprot:
  • NP_002070.1(Human)