Mouse ASK1 ELISA Kit (Colorimetric)

ELISA: Mouse ASK1 ELISA Kit (Colorimetric) [NBP2-69953] - Samples were spiked with high concentrations of Mouse ASK1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse ASK1 ELISA Kit (Colorimetric) [NBP2-69953] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL

Mouse ASK1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse ASK1 in samples. No significant cross-reactivity or interference between Mouse ASK1 and analogues was observed.
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.81%
• Intra-Assay: CV% < 5.71%
• Spike Recovery: 89-104%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: MAP3K5

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse ASK1 ELISA Kit (Colorimetric)

• Apoptosis signal-regulating kinase 1
• ASK1
• ASK-1
• ASK1MEKK 5
• EC 2.7.11
• MAP/ERK kinase kinase 5
• MAP3K5
• MAPK/ERK kinase kinase 5
• MAPKKK5EC 2.7.11.25
• MEK kinase 5
• MEKK5
• MEKK5apoptosis signal regulating kinase 1
• mitogen-activated protein kinase kinase kinase 5

Background

Apoptosis signal-regulating kinase-1 (ASK1, MEKK5, MAPKKK5) is a MAP3k type protein kinase belonging to mitogen-activated protein kinase kinase kinase (MAPKKK) family. As a regulator of JNK and p38 MAPK signaling pathways, ASK1/MEKK5 phosphorylates MMK4 to activate c-Jun N-terminal kinase (JNK) and phosphorylates MEK3 and MKK6 to activate p38. ASK1/MEKK5 is activated by oxidative stress, TNF via TRAF2, Fas via Daxx, calcium overload, and endoplasmic reticulum stress (1). ASK1/MEKK5 is inactivated by Trx, Grx, 14-3-3, and protein serine/threonine phosphatase 5 (2). While elevated level of ASK1/MEKK5 can induce apoptosis, a catalytically inactive ASK1/MEKK5 acts as an inhibitor in TNF-alpha induced apoptosis. Over-expression of ASK1/MEKK5 has also been implicated in cell differentiation, such as in keratinocyte differentiation through p38 pathway (3). ASK1/MEKK5 has been implicated as a therapeutic target for neurodegenerative disorder sand cardiac dysfunction (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for ASK1 ELISA Kit (NBP2-69953)(1)

Publication using NBP2-69953

• Valuparampil Varghese M, James J, Bharti D et al. Circulating free heme induces cytokine storm and pulmonary hypertension through the MKK3/p38 axis American Journal of Physiology - Lung Cellular and Molecular Physiology 2024-10-01 [PMID: 39197168]

FAQs for ASK1 ELISA Kit (NBP2-69953). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: MAP3K5