ASIP Antibody

Product Discontinued

ASIP Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SHLPPEEKLRDDRSLRSNSSVNLLDVPSVSIVALNKKSKQIGRKAAEKKR SSKKEA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ASIP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Use in Western blot reported in scientific literature (PMID: 29559925).

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 29559925).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ASIP Antibody

• agouti (mouse)-signaling protein
• agouti signaling protein
• agouti signaling protein, nonagouti homolog (mouse)
• agouti signaling protein, nonagouti homolog
• Agouti switch protein
• agouti-signaling protein
• AGSW
• AGTI
• AGTIL
• AGTILMGC126092
• ASIP
• ASPAGSW
• MGC126093
• nonagouti homolog
• SHEP9

Background

The agouti signaling protein (ASIP) is an adaptor protein involved in asymmetrical cell division and cell polarization processes. ASIP seems to play a central role in the formation of epithelial tight junctions. ASIP may affect the quality of hair pigmentation, and act as a pharmacological antagonist of alpha-melanocyte-stimulating hormone (2-3). ASIP may also play a role in neuroendocrine aspects of melanocortin action, and have a functional role in regulating lipid metabolism in adipocytes (4-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ASIP Antibody (NBP2-14323)(1)

Publication using NBP2-14323

• Liu Y, Albrecht E, Schering L et al. Agouti Signaling Protein and Its Receptors as Potential Molecular Markers for Intramuscular and Body Fat Deposition in Cattle. Front Physiol. 2018-03-06 [PMID: 29559925] (WB, Bovine)
  
  Details:
  The Novus ASIP anti-human antibody was tested on bovine samples, no cross-reactivity was observed

Bioinformatics

• Gene Symbol: ASIP