ASH2L Antibody (ST47-01)

Western Blot: ASH2L Antibody (ST47-01) [NBP2-67500] - Analysis of ASH2L on different lysates using anti-ASH2L antibody at 1/1,000 dilution. Positive control: Lane 1: PC12 Lane 2: 293T

Immunocytochemistry/Immunofluorescence: ASH2L Antibody (ST47-01) [NBP2-67500] - Staining ASH2L in CRC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ASH2L Antibody (ST47-01) [NBP2-67500] - Analysis of paraffin-embedded mouse testis tissue using anti-ASH2L antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: ASH2L Antibody (ST47-01) [NBP2-67500] - Staining ASH2L in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: ASH2L Antibody (ST47-01) [NBP2-67500] - Staining ASH2L in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ASH2L Antibody (ST47-01) [NBP2-67500] - Analysis of paraffin-embedded mouse colon tissue using anti-ASH2L antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ASH2L Antibody (ST47-01) [NBP2-67500] - Analysis of paraffin-embedded mouse ovary tissue using anti-ASH2L antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ASH2L Antibody (ST47-01) [NBP2-67500] - Analysis of paraffin-embedded mouse placenta tissue using anti-ASH2L antibody. Counter stained with hematoxylin.

Product Discontinued

ASH2L Antibody (ST47-01) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human ASH2L aa 533-628 / 628. (SwissProt: Q9UBL3 Human; SwissProt: Q91X20 Mouse; Unigene: 219095 Rat)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ASH2L
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ASH2L Antibody (ST47-01)

• ash2 (absent, small, or homeotic)-like (Drosophila)
• ASH2
• ASH2L1ash2 (absent, small, or homeotic, Drosophila, homolog)-like
• ASH2L2ASH2-like protein
• Bre2
• set1/Ash2 histone methyltransferase complex subunit ASH2

Background

ASH2L is a component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. May function as a transcriptional regulator. May play a rol

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ASH2L