ARL4 Antibody

Western Blot: ARL4 Antibody [NB100-1080] - (2ug/ml) of Human Testis lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ARL4 Antibody Summary

• Immunogen: Peptide with sequence GNGLSDQTSILSN-C corresponding to N-Terminus according to NP_001032241.1, NP_005729.1, NP_997625.1.
• Specificity: This is also expected to react with a hypothetical human protein, XP_166703, and a hypothetical mouse protein, AAH03725 - both very similar to ARL4.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARL4A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 23 kDa band observed in human testis lysate.
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  ARL4 Overexpression Lysate
  Human Testis Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARL4 Antibody

• ADP-ribosylation factor-like 4A
• ADP-ribosylation factor-like protein 4A
• ARL4ADP-ribosylation factor-like 4

Background

ADP-ribosylation factors (ARFs) regulate diverse cellular functions, such as vesicle traffic, endocytosis, and phospholipase D activity. ARFs and ARF-like (ARL) proteins, which share sequence similarity with ARFs, form a subfamily of the Ras-related GTPase superfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARL4 Antibody (NB100-1080)(1)

Publication using NB100-1080

• Jacobs S, Schurmann A, Becker W et al. The mouse ADP-ribosylation factor-like 4 gene: two separate promoters direct specific transcription in tissues and testicular germ cell. Biochem J 1998-10-15 [PMID: 9761722]

Bioinformatics

• Gene Symbol: ARL4A
• Entrez:
  • 10124(Human)
  • 11861(Mouse)
• Uniprot:
  • P40617(Human)