Argonaute 3 Antibody (PCRP-AGO3-1C5)

Immunocytochemistry/Immunofluorescence: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873] - Immunofluorescence Analysis of PFA-fixed HeLa cells. Cytoplasmic P-bodies and nucleoplasm stained using Argonaute 3 Antibody (PCRP-AGO3-1C5) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Flow Cytometry: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873] - Flow cytometric analysis of PFA-fixed HeLa cells. Argonaute 3 antibody (PCRP-AGO3-1C5) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Protein Array: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Argonaute 3 Antibody (PCRP-AGO3-1C5).

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IP, MA
• Clone: PCRP-AGO3-1C5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Argonaute 3 Antibody (PCRP-AGO3-1C5) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14202)
  
  Antibody with azide store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human Argonaute 3 protein (Uniprot: Q9H9G7)
• Localization: Cytoplasm, P-bodies, Nucleoplasm
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: AGO3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
• Application Notes: ELISA: For coating, order antibody without BSA
  
  Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
• Theoretical MW: 97.36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Argonaute 3 Antibody (PCRP-AGO3-1C5)

• AGO3argonaute 3
• argonaute3
• eIF2C 3
• eIF-2C 3
• Eukaryotic translation initiation factor 2C 3
• eukaryotic translation initiation factor 2C, 3
• FLJ12765
• hAgo3
• MGC86946
• protein argonaute-3

Background

Argonaute 3 encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AGO3
• Uniprot:
  • Q9H9G7(Human)