ARFIP2 Antibody (2B5) - Azide and BSA Free

Western Blot: ARFIP2 Antibody (2B5) [H00023647-M01] - ARFIP2 monoclonal antibody (M01), clone 2B5 Analysis of ARFIP2 expression in IMR-32.

Sandwich ELISA: ARFIP2 Antibody (2B5) [H00023647-M01] - Detection limit for recombinant GST tagged ARFIP2 is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ARFIP2 Antibody (2B5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ARFIP2 Antibody (2B5) - Azide and BSA Free (H00023647-M01) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-ARFIP2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: ARFIP2 (AAH00392, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTDGILGKAATMEIPIHGNGEARQLPEDDGLEQDLQQVMVSGPNLNETSIVSGGYGGSGDGLIPTGSGRHPSHSTTPSGPGDEVARGIAGEKFDIVKKWGINTYKCTKQL
• Localization: Cytoplasmic
• Specificity: ARFIP2 - ADP-ribosylation factor interacting protein 2 (arfaptin 2)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARFIP2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ARFIP2 Antibody (2B5) - Azide and BSA Free

• ADP-ribosylation factor interacting protein 2
• ADP-ribosylation factor-interacting protein 2
• arfaptin 2
• arfaptin-2
• Partner of RAC1
• POR1partner of RAC1 (arfaptin 2)
• Protein POR1

Background

ARFIP2 is a ubiquitously expressed protein implicated in mediating cross talk between RAC and ARF small GTPases. It has been shown that ARFIP2 binds specifically to GTP-bound ARF1 and ARF6, but binds to Rac-GTP and Rac-GDP with similar affinities. The X-ray structure of arfaptin reveals an elongated, crescent-shaped dimer of 3-helix coiled-coils. Structures of arfaptin with Rac bound to either GDP or the slowly hydrolysable analog GMPPNP show that the switch regions adopt similar conformations in both complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARFIP2 Antibody (H00023647-M01)(2)

Publications using H00023647-M01

• Cruz-Garcia D, Ortega-Bellido M, Scarpa M et al. Recruitment of arfaptins to the trans-Golgi network by PI(4)P and their involvement in cargo export. EMBO J. 2013-05-21 [PMID: 23695357]
• Man Z, Kondo Y, Koga H et al. Arfaptins Are Localized to the trans-Golgi by Interaction with Arl1, but Not Arfs. J Biol Chem. 2011-01-14 [PMID: 21239483]

Bioinformatics

• Gene Symbol: ARFIP2
• Entrez:
  • 23647(Human)
• OMIM:
  • 601638(Human)
• Uniprot:
  • AAH00392(Human)