ARFGAP1 Antibody (OTI1F6) - Azide and BSA Free

Western Blot: ARFGAP1 Antibody (OTI1F6) - Azide and BSA Free [NBP2-72013] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARFGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARFGAP1.

Immunocytochemistry/Immunofluorescence: ARFGAP1 Antibody (OTI1F6) - Azide and BSA Free [NBP2-72013] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ARFGAP1.

Immunohistochemistry: ARFGAP1 Antibody (OTI1F6) - Azide and BSA Free [NBP2-72013] - Staining of paraffin-embedded Human pancreas tissue using anti-ARFGAP1 mouse monoclonal antibody.

Flow Cytometry: ARFGAP1 Antibody (OTI1F6) - Azide and BSA Free [NBP2-72013] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-ARFGAP1 antibody, and then analyzed by flow cytometry.

Western Blot: ARFGAP1 Antibody (OTI1F6) - Azide and BSA Free [NBP2-72013] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ARFGAP1 monoclonal antibody.

Immunohistochemistry: ARFGAP1 Antibody (OTI1F6) - Azide and BSA Free [NBP2-72013] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ARFGAP1 mouse monoclonal antibody.

Immunohistochemistry: ARFGAP1 Antibody (OTI1F6) - Azide and BSA Free [NBP2-72013] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ARFGAP1 mouse monoclonal antibody.

Immunohistochemistry: ARFGAP1 Antibody (OTI1F6) - Azide and BSA Free [NBP2-72013] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ARFGAP1 mouse monoclonal antibody.

Immunohistochemistry: ARFGAP1 Antibody (OTI1F6) - Azide and BSA Free [NBP2-72013] - Staining of paraffin-embedded Human endometrium tissue using anti-ARFGAP1 mouse monoclonal antibody.

Immunohistochemistry: ARFGAP1 Antibody (OTI1F6) - Azide and BSA Free [NBP2-72013] - Staining of paraffin-embedded Human Kidney tissue using anti-ARFGAP1 mouse monoclonal antibody.

Immunohistochemistry: ARFGAP1 Antibody (OTI1F6) - Azide and BSA Free [NBP2-72013] - Staining of paraffin-embedded Human liver tissue using anti-ARFGAP1 mouse monoclonal antibody.

Immunohistochemistry: ARFGAP1 Antibody (OTI1F6) - Azide and BSA Free [NBP2-72013] - Staining of paraffin-embedded Human lymphoma tissue using anti-ARFGAP1 mouse monoclonal antibody.

Immunohistochemistry: ARFGAP1 Antibody (OTI1F6) - Azide and BSA Free [NBP2-72013] - Staining of paraffin-embedded Human thyroid tissue using anti-ARFGAP1 mouse monoclonal antibody.

Flow Cytometry: ARFGAP1 Antibody (OTI1F6) - Azide and BSA Free [NBP2-72013] - Analysis of Hela cells, using anti-ARFGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

CyTOF-ready: ARFGAP1 Antibody (OTI1F6) - Azide and BSA Free [NBP2-72013] - Analysis of Jurkat cells, using anti-ARFGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI1F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ARFGAP1 Antibody (OTI1F6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ARFGAP1 Antibody (OTI1F6) - Azide and BSA Free (NBP2-00513) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human ARFGAP1 (NP_783202) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARFGAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-2000
• Theoretical MW: 45.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for ARFGAP1 Antibody (OTI1F6) - Azide and BSA Free

• ADP-ribosylation factor 1 GTPase activating protein
• ADP-ribosylation factor 1 GTPase-activating protein
• ADP-ribosylation factor GTPase activating protein 1
• ADP-ribosylation factor GTPase-activating protein 1
• ARF GAP 1
• ARF1 GAP
• ARF1-directed GTPase-activating protein
• ARF1GAP
• ARFGAP1
• bA261N11.3
• FLJ10767
• GAP protein
• HRIHFB2281
• MGC39924

Background

ARFGAP1 is encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARFGAP1