ARFGAP1 Antibody (1C4E2) - BSA Free

Western Blot: ARFGAP1 Antibody (1C4E2) [NBP2-61670] - Analysis using ARFGAP1 mAb against HEK293 (1) and ARFGAP1 (AA: 270-414)-hIgGFc transfected HEK293 (2) cell lysate.

Flow Cytometry: ARFGAP1 Antibody (1C4E2) [NBP2-61670] - Analysis of HepG2 cells using ARFGAP1 mouse mAb (green) and negative control (red).

Western Blot: ARFGAP1 Antibody (1C4E2) [NBP2-61670] - Analysis using ARFGAP1 mAb against human ARFGAP1 (AA: 270-414) recombinant protein. (Expected MW is 41.5 kDa)

Western Blot: ARFGAP1 Antibody (1C4E2) [NBP2-61670] - Analysis using ARFGAP1 mouse mAb against MOLT4 (1), C2C12 (2), HepG2 (3), MCF-7 (4), and Lncap (5) cell lysate.

ELISA: ARFGAP1 Antibody (1C4E2) [NBP2-61670] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow
• Clone: 1C4E2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ARFGAP1 Antibody (1C4E2) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human ARFGAP1 (AA: 270-414) expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARFGAP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Western Blot 1:100-1:2000
• Theoretical MW: 44.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for ARFGAP1 Antibody (1C4E2) - BSA Free

• ADP-ribosylation factor 1 GTPase activating protein
• ADP-ribosylation factor 1 GTPase-activating protein
• ADP-ribosylation factor GTPase activating protein 1
• ADP-ribosylation factor GTPase-activating protein 1
• ARF GAP 1
• ARF1 GAP
• ARF1-directed GTPase-activating protein
• ARF1GAP
• ARFGAP1
• bA261N11.3
• FLJ10767
• GAP protein
• HRIHFB2281
• MGC39924

Background

The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARFGAP1