Recombinant Human ARF4 Protein

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SDS-Page: Recombinant Human ARF4 Protein [NBP1-50948] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human ARF4 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-180 of Human ARF4

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMGLTIS SLFSRLFGKK QMRILMVGLD AAGKTTILYK LKLGEIVTTI PTIGFNVETV EYKNICFTVW DVGGQDRIRP LWKHYFQNTQ GLIFVVDSND RERIQEVADE LQKMLLVDEL RDAVLLLFAN KQDLPNAMAI SEMTDKLGLQ SLRNRTWYVQ ATCATQGTGL YEGLDWLSNE LSKR

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
ARF4
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0) containing 0.2M Nacl, 5mM DTT, 20% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human ARF4 Protein

  • ADP-ribosylation factor 4
  • ARF2ADP-ribosylation factor 2

Background

ARF4 is a member of the ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The ARF proteins include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. They are categorized as class I, class II and class III; this gene is a class II member. Recombinant human ARF4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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FAQs for ARF4 Protein (NBP1-50948). (Showing 1 - 1 of 1 FAQ).

  1. I was looking for antibodies against human ARF4 and ARF5 and I need to be able to distinguish between the two proteins. Do you have antibodies specific for each that don't cross react with the other protein?
    • The sequence similarity between ARF4 and ARF5 shows very high homology between the two proteins. We have not specifically tested for cross-reactivity between the antibodies, but I think it's very likely that they will cross react.

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Bioinformatics

Gene Symbol ARF4