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Aquaporin-3 Antibody

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Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Aquaporin-3 Antibody Summary

Immunogen
A synthetic peptide from the c-terminal region of rat Aquaporin 3 (AQP3) conjugated to an immunogenic carrier protein was used as the antigen.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AQP3
Purity
Unpurified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1 : 300 - 1 : 2000
  • Immunohistochemistry-Paraffin 1: 300 - 1:2000
  • Western Blot 1:300-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Concentration
LYOPH
Purity
Unpurified
Reconstitution Instructions
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability.

Notes

This peptide is soluble in PBS and water.

Alternate Names for Aquaporin-3 Antibody

  • AQP-3
  • aquaglyceroporin-3
  • aquaporin 3 (GIL blood group)
  • aquaporin 3 (Gill blood group)
  • aquaporin 3
  • aquaporin-3
  • GIL
  • Gill blood group

Background

AQP3 is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Aquaporin-3 Antibody (NBP1-70376). (Showing 1 - 1 of 1 FAQ).

  1. I am from China and I would like to know more about your product number:NBL1-07638. We know that the theoretical MW of AQP3 protein is 31KD. But in your product specification, we can see that so many bands appear after overexpressing AQP3 with plasmid in 293 cell. Can you explain this phenomenon? Apart from theoretical strip, are all other nonspecific bands? Or is the AQP3 itself ? 
    • Yes, the theoretical size of AQP3 is 31 kDa, which would be expected under reduced conditions with a sample. We have not characterized any of the other bands in the overexpressed lysate lane. Because this is a native lysate, any modifications that may take place could cause smearing and extra bands in the lysate sample. AQP3 is extensively modified because it is a glycoprotein, glycosylation often shows up as multiple bands with smearing when run under native conditions.  This overexpression lysate is meant to only act as a positive control in WB experiments requiring a native control. Furthermore, the lane on the right side of the blot is showing detection of the lysate using an anti-Flag tag antibody, since the construct used for overexpression contains a Flag tag. 

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Aquaporin-3 Antibody (NBP1-70376)

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Bioinformatics

Gene Symbol AQP3