Aquaporin 1/AQP1 Overexpression Lysate (Native)

Images

 
Western Blot: Aquaporin-1 Overexpression Lysate (Adult Normal) [NBL1-07635] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Aquaporin-1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Aquaporin 1/AQP1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205304 and based on accession number NM_198098. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens aquaporin 1 (Colton blood group) (AQP1), mRNA.
Gene
AQP1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
28.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Aquaporin 1/AQP1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Aquaporin 1/AQP1 Overexpression Lysate (Native)

  • 28-kDa
  • AQP1
  • AQP-1
  • AQP-CHIP
  • aquaporin 1 (channel-forming integral protein, 28kDa)
  • aquaporin 1 (Colton blood group)
  • Aquaporin 1
  • Aquaporin-CHIP
  • CHIP28
  • CHIP2828kDa, CO blood group)
  • CO
  • MGC26324

Background

Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). This gene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayer spanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant in erythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involving imbalance in ocular fluid movement. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-87679
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB110-74682
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-33872
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-39043
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-30862
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr
NBP2-58661
Species: Hu
Applications: IHC-P
NBP1-30865
Species: Hu
Applications: WB, IHC, IHC-P
NB100-65214
Species: Hu, Mu, Rt, Ma
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
H00000343-M01
Species: Hu, Rt
Applications: WB, ELISA
NBP1-57622
Species: Hu
Applications: WB, IHC, IHC-P
NB100-77316
Species: Hu, Mu
Applications: WB (-), ICC/IF, IHC, IHC-P, IP
AF2030
Species: Hu
Applications: WB, IHC
NBL1-07635
Species: Hu
Applications: WB

Publications for Aquaporin 1/AQP1 Lysate (NBL1-07635) (0)

There are no publications for Aquaporin 1/AQP1 Lysate (NBL1-07635).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Aquaporin 1/AQP1 Lysate (NBL1-07635) (0)

There are no reviews for Aquaporin 1/AQP1 Lysate (NBL1-07635). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Aquaporin 1/AQP1 Lysate (NBL1-07635). (Showing 1 - 1 of 1 FAQ).

  1. Are any of your Aquaporin-1 antibodies suitable for live cell staining? I am looking at doing flow cytometry on live cells and further isolation of the positive cells to purify the population.
    • A list of our Aquaporin-1 antibodies suitable for use in flow cytometry can be found using this link. Of note, NB600-741 is known to bind to an extracellular domain, so should be suitable for your use.

Additional Aquaporin 1/AQP1 Products

Bioinformatics Tool for Aquaporin 1/AQP1 Lysate (NBL1-07635)

Discover related pathways, diseases and genes to Aquaporin 1/AQP1 Lysate (NBL1-07635). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Aquaporin 1/AQP1 Lysate (NBL1-07635)

Discover more about diseases related to Aquaporin 1/AQP1 Lysate (NBL1-07635).
 

Pathways for Aquaporin 1/AQP1 Lysate (NBL1-07635)

View related products by pathway.

PTMs for Aquaporin 1/AQP1 Lysate (NBL1-07635)

Learn more about PTMs related to Aquaporin 1/AQP1 Lysate (NBL1-07635).

Blogs on Aquaporin 1/AQP1

There are no specific blogs for Aquaporin 1/AQP1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

GM-CSF [Unconjugated]
215-GM-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Aquaporin 1/AQP1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol AQP1
Entrez