22.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NBP1-28870 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified
Alternate Names for APOO Antibody (2F1)
apolipoprotein O
FAM121Bbrain my025
family with sequence similarity 121B
MGC4825
My025
MYO25
Protein FAM121B
Background
apolipoprotein O, also known as MYO25, FAM121B, MGC4825. Entrez Protein NP_077027. It is a chrondroitin-sulfate chain containing member of the apolipoprotein family and is an original glycoprotein up-regulated by diabetes in human Heart. Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL. May be involved in myocardium-protective mechanisms against lipid accumulation.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Bioinformatics Tool for APOO Antibody (NBP1-28870)
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Diseases for APOO Antibody (NBP1-28870)
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.
![Western Blot: APOO Antibody (2F1) [NBP1-28870] - Analysis using ApoO mouse mAb against HepG2 (1) and 3T3L1(2) cell lysate.](http://images.novusbio.com/fullsize2/APOO-Antibody-(2F1)-Western-Blot-NBP1-28870-img0002.jpg "Western Blot: APOO Antibody (2F1) [NBP1-28870] - Analysis using ApoO mouse mAb against HepG2 (1) and 3T3L1(2) cell lysate.")
![Immunohistochemistry-Paraffin: APOO Antibody (2F1) [NBP1-28870] - Analysis of human Testis tissues using ApoO mouse mAb.](http://images.novusbio.com/fullsize2/APOO-Antibody-(2F1)-Immunohistochemistry-Paraffin-NBP1-28870-img0001.jpg "Immunohistochemistry-Paraffin: APOO Antibody (2F1) [NBP1-28870] - Analysis of human Testis tissues using ApoO mouse mAb.")
![Western Blot: Guanine deaminase Antibody [NBP1-87525] - Analysis in control (vector only transfected HEK293T lysate) and GDA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize2/Guanine-deaminase-Antibody-Western-Blot-NBP1-87525-img0008.jpg)
![Immunohistochemistry-Paraffin: Guanine deaminase Antibody [NBP1-87525] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize2/Guanine deaminase Antibody-Immunohistochemistry-Paraffin-NBP1-87525-img0011.jpg)
![Immunohistochemistry-Paraffin: Guanine deaminase Antibody [NBP1-87525] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize2/Guanine-deaminase-Antibody-Immunohistochemistry-Paraffin-NBP1-87525-img0005.jpg)
![Western Blot: KCNIP4 Antibody [NBP2-84105] - WB Suggested Anti-KCNIP4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate](http://images.novusbio.com/fullsize2/KCNIP4 Antibody-Western Blot-NBP2-84105-img0002.jpg)
![Immunohistochemistry: KCNIP4 Antibody [NBP2-84105] - Human Brain](http://images.novusbio.com/fullsize2/KCNIP4-Antibody-Immunohistochemistry-NBP2-84105-img0001.jpg)
![Western Blot: Elp3 Antibody (11811) [NBP2-43694] - Analysis of various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELP3 antibody diluted by 1:500.](http://images.novusbio.com/fullsize2/Elp3-Antibody-(11811)-Western-Blot-NBP2-43694-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Elp3 Antibody (11811) [NBP2-43694] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ELP3 protein stained by ELP3 antibody [11811] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize2/Elp3-Antibody-(11811)-Immunocytochemistry-Immunofluorescence-NBP2-43694-img0003.jpg)
![Western Blot: Elp3 Antibody (11811) [NBP2-43694] - Analysis of HeLa whole cell extracts and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELP3 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize2/Elp3-Antibody-(11811)-Western-Blot-NBP2-43694-img0001.jpg)
![Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - Total protein from HepG2 and Hek293 cells was separated on a 4-15% gradient gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-APOA1 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/Apolipoprotein-A-I-ApoA1-Antibody-Western-Blot-NBP2-52979-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-APOA1 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/Apolipoprotein-A-I-ApoA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52979-img0001.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse liver using APOA1 antibody at 1:400 dilution. The antibody generated a weak/mild cytoplasmic signal in hepatocytes with a very strong positivity near the hepatocyte membranes as well as in the inter-cellular spaces.](http://images.novusbio.com/fullsize2/Apolipoprotein-A-I-ApoA1-Antibody-Immunohistochemistry-Paraffin-NBP2-52979-img0002.jpg)
![Western Blot: PP14/Glycodelin Antibody [NBP1-89781] - Analysis in human placenta tissue.](http://images.novusbio.com/fullsize2/PP14-Glycodelin-Antibody-Western-Blot-NBP1-89781-img0014.jpg)
![Immunohistochemistry-Paraffin: PP14/Glycodelin Antibody [NBP1-89781] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize2/PP14-Glycodelin-Antibody-Immunohistochemistry-Paraffin-NBP1-89781-img0019.jpg)
![Western Blot: PP14/Glycodelin Antibody [NBP1-89781] - Analysis in control (vector only transfected HEK293T lysate) and PAEP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize2/PP14-Glycodelin-Antibody-Western-Blot-NBP1-89781-img0009.jpg)
![Western Blot: GDC Antibody [NBP1-91933] - Analysis in control (vector only transfected HEK293T lysate) and SLC25A16 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize2/GDC Antibody-Western Blot-NBP1-91933-img0003.jpg)
![Immunohistochemistry-Paraffin: GDC Antibody [NBP1-91933] - Staining of human pancreas shows distinct cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize2/GDC-Antibody-Immunohistochemistry-Paraffin-NBP1-91933-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Thioredoxin at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/Thioredoxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52983-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Thioredoxin at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/Thioredoxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52983-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Thioredoxin at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/Thioredoxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52983-img0006.jpg)
![Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Sample : FFPE section of prostate adenocarcinoma. Antibody : Affinity purified rabbit anti-NCOA2/SRC2 used at a dilution of 1:100. Detection : DAB](http://images.novusbio.com/fullsize2/NCOA2-Antibody-Immunohistochemistry-NB100-1756-img0003.jpg)
![Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Samples: FFPE sections of human breast adenocarcinoma (upper image) and prostate adenocarcinoma (lower image). Antibody: Affinity purified rabbit anti-SRC2 NB100-1756 used at a dilution of 1:100. Detection: DAB](http://images.novusbio.com/fullsize2/NCOA2-Antibody-Immunohistochemistry-NB100-1756-img0001.jpg)
![Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Sample : FFPE section of human breast adenocarcinoma. Antibody : Affinity purified rabbit anti-NCOA2/SRC2 used at a dilution of 1:250. Detection : DAB](http://images.novusbio.com/fullsize2/NCOA2-Antibody-Immunohistochemistry-NB100-1756-img0002.jpg)
![Western Blot: GRIP1 Antibody [NBP2-41211] - Analysis of GRIP1 in 293 cell lysate with GRIP1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize2/GRIP1-Antibody-Western-Blot-NBP2-41211-img0004.jpg)
![Immunohistochemistry: GRIP1 Antibody [NBP2-41211] - Immunohistochemistry of GRIP1 in rat brain tissue with GRIP1 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize2/GRIP1-Antibody-Immunohistochemistry-NBP2-41211-img0002.jpg)
![Immunofluorescence: GRIP1 Antibody [NBP2-41211] - Immunofluorescence of GRIP1 in rat brain tissue with GRIP1 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize2/GRIP1-Antibody-Immunofluorescence-NBP2-41211-img0003.jpg)
![Western Blot: LGALS12 Antibody [H00085329-B02P] - Analysis of LGALS12 expression in transfected 293T cell line by LGALS12 polyclonal antibody. Lane 1: LGALS12 transfected lysate(37.50 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/LGALS12 Antibody-Western Blot-H00085329-B02P-img0001.jpg)
![Western Blot: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Analysis of Rat brain membrane lysate showing detection of Ankyrin G protein using Mouse Anti-Ankyrin G Monoclonal Antibody, Clone S106-20. Primary Antibody: Mouse Anti-Ankyrin G Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize2/Ankyrin-3-Antibody-(S106-20)-Western-Blot-NBP2-59310-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin G Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X.](http://images.novusbio.com/fullsize2/Ankyrin-3-Antibody-(S106-20)-Immunocytochemistry-Immunofluorescence-NBP2-59310-img0002.jpg)
![Western Blot: Acetyl CoA synthetase Antibody [NBP1-90276] - Analysis in human cell line RPMI-8226.](http://images.novusbio.com/fullsize2/Acetyl-CoA-synthetase-Antibody-Western-Blot-NBP1-90276-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Acetyl CoA synthetase Antibody [NBP1-90276] - Staining of human cell line A-431 shows localization to nucleoplasm, cytosol & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/Acetyl CoA synthetase Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90276-img0010.jpg)
![Immunohistochemistry-Paraffin: Acetyl CoA synthetase Antibody [NBP1-90276] - Staining of human tonsil shows no positivity in lymphocytes as expected.](http://images.novusbio.com/fullsize2/Acetyl-CoA-synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-90276-img0014.jpg)
![Western Blot: LYPLA3 Antibody [NBP1-92088] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize2/LYPLA3-Antibody-Western-Blot-NBP1-92088-img0006.jpg)
![Immunohistochemistry-Paraffin: LYPLA3 Antibody [NBP1-92088] - Staining of human lymph node.](http://images.novusbio.com/fullsize2/LYPLA3 Antibody-Immunohistochemistry-Paraffin-NBP1-92088-img0013.jpg)
![Western Blot: LYPLA3 Antibody [NBP1-92088] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize2/LYPLA3-Antibody-Western-Blot-NBP1-92088-img0004.jpg)
![Western Blot: Papilin Antibody (5B2E5) [NBP2-37320] - Western blot analysis using PAPLN mouse mAb against Hela (1), HepG2 (2), OCM-1 (3), Raji (4), Jurkat (5), NIH/3T3 (6) cell lysate.](http://images.novusbio.com/fullsize2/Papilin-Antibody-(5B2E5)-Western-Blot-NBP2-37320-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Papilin Antibody (5B2E5) [NBP2-37320] - Immunofluorescence analysis of NIH/3T3 cells using PAPLN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize2/Papilin-Antibody-(5B2E5)-Immunocytochemistry-Immunofluorescence-NBP2-37320-img0005.jpg)
![Western Blot: Papilin Antibody (5B2E5) [NBP2-37320] - Western blot analysis using PAPLN mAb against human PAPLN recombinant protein. (Expected MW is 36.4 kDa)](http://images.novusbio.com/fullsize2/Papilin-Antibody-(5B2E5)-Western-Blot-NBP2-37320-img0002.jpg)
![Western Blot: alpha-2A Adrenergic R/ADRA2A Antibody [NBP2-22452] - Analysis was performed on whole cell extracts (30 ug lysate) of PC-3 (Lane 1), HEK-293 (Lane 2), T47D (Lane 3) and HEL 92.1.7 (lane 4). The blots were probed with Anti-alpha-2a Adrenergic Receptor Rabbit Polyclonal Antibody.](http://images.novusbio.com/fullsize2/alpha-2A-Adrenergic-R-ADRA2A-Antibody-Western-Blot-NBP2-22452-img0001.jpg)
![SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](http://images.novusbio.com/images2/Mouse-IgG1-Isotype-Control-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")