APOO Antibody (2F1)

Western Blot: APOO Antibody (2F1) [NBP1-28870] - Analysis using ApoO mouse mAb against HepG2 (1) and 3T3L1(2) cell lysate.

Immunohistochemistry-Paraffin: APOO Antibody (2F1) [NBP1-28870] - Analysis of human Testis tissues using ApoO mouse mAb.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clone: 2F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

APOO Antibody (2F1) Summary

• Immunogen: Purified recombinant fragment of APOO expressed in E. Coli.
• Specificity: Specific for ApoO.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: APOO
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 22.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for APOO Antibody (2F1)

• apolipoprotein O
• FAM121Bbrain my025
• family with sequence similarity 121B
• MGC4825
• My025
• MYO25
• Protein FAM121B

Background

apolipoprotein O, also known as MYO25, FAM121B, MGC4825. Entrez Protein NP_077027. It is a chrondroitin-sulfate chain containing member of the apolipoprotein family and is an original glycoprotein up-regulated by diabetes in human Heart. Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL. May be involved in myocardium-protective mechanisms against lipid accumulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APOO Antibody (NBP1-28870)(3)

Publications using NBP1-28870

• Liu T, Woo JA, Bukhari MZ et al. CHCHD10-regulated OPA1-mitofilin complex mediates TDP-43-induced mitochondrial phenotypes associated with frontotemporal dementia FASEB J. 2020-05-05 [PMID: 32369233] (Mouse)
• Guarani V, Jardel C, Chretien D et al. QIL1 mutation causes MICOS disassembly and early onset fatal mitochondrial encephalopathy with liver disease Elife 2016-09-14 [PMID: 27623147] (WB, Human)
• Guarani V, McNeill EM, Paulo JA et al. QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology. Elife. 2015-05-22 [PMID: 25997101] (Human)

FAQs for APOO Antibody (NBP1-28870). (Showing 1 - 1 of 1 FAQs).

1. Do you have the sequence used as antigen?
   • The immunogen range for our product NBP1-28870 is aa26-198 of the human protein.

Bioinformatics

• Gene Symbol: APOO