Elp3 Antibody (11811) - Azide and BSA Free

Western Blot: Elp3 Antibody (11811) [NBP2-43694] - Analysis of various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELP3 antibody diluted by 1:500.

Immunocytochemistry/Immunofluorescence: Elp3 Antibody (11811) [NBP2-43694] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ELP3 protein stained by ELP3 antibody [11811] diluted at 1:500. Blue: Hoechst 33342 staining.

Western Blot: Elp3 Antibody (11811) [NBP2-43694] - Analysis of HeLa whole cell extracts and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELP3 antibody diluted by 1:1000.

Immunoprecipitation: Elp3 Antibody (11811) [NBP2-43694] - Analysis of ELP3 protein from 293T whole cell extracts using 5 ug of ELP3 antibody [11811]. Western blot analysis was performed using ELP3 antibody [11811]. EasyBlot anti-Mouse IgG was used as a secondary reagent.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clone: 11811
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Elp3 Antibody (11811) - Azide and BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Elp3. The exact sequence is proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ELP3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 62 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for Elp3 Antibody (11811) - Azide and BSA Free

• EC 2.3.1.48
• elongation protein 3 homolog (S. cerevisiae)
• elongator complex protein 3
• hELP3
• KAT9FLJ10422

Background

ELP3 is the catalytic subunit of the histone acetyltransferase elongator complex, which contributes to transcript elongation and also regulates the maturation of projection neurons (Creppe et al., 2009 (PubMed 19185337)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ELP3