Recombinant Human Apolipoprotein F GST (N-Term) Protein

SDS-Page: Recombinant Human Apolipoprotein F Protein [H00000319-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

• Reactivity: Hu
• Applications: WB, ELISA, MA, AP, MS

Recombinant Human Apolipoprotein F GST (N-Term) Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-291 of Human APOF
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: TSYGKQTNVLMHFPLSLESQTPSSDPLSCQFLHPKSLPGFSHMAPLPKFLVSLALRNDALEEAGCQADVWALQLQLYRQGGVNATQVLIQHLRGLQKGRSTERNVSVEALASALQLLAREQQSTGRVGRSLPTEDCENEKEQAVHNVVQLLPGVGTFYNLGTALYYATQNCLGKARERGRDGAIDLGYDLLMTMAGMSGGPMGLAISAALKPALRSGVQQLIQYYQDQKDANISQPETTKEGLRAISDVSDLEETTTLASFISEVVSSAPYWGWAIIKSYDLDPGAGSLEI

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: APOF
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Mass Spectrometry
  • Protein Array
  • Western Blot
• Theoretical MW: 57.64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human Apolipoprotein F GST (N-Term) Protein

• Apo-F
• apolipoprotein F
• DKFZp781G18150
• Lipid transfer inhibitor protein
• LTIP
• MGC22520

Background

APOF( AAH26257, 37 a.a. - 327 a.a.) recombinant protein with GST.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Apolipoprotein F Recombinant Protein (H00000319-P01)(1)

Publication using H00000319-P01

• Abhinav Kumar A, Gangadharan B, Zitzmann N. Multiple reaction monitoring and multiple reaction monitoring cubed based assay for the quantitation of apolipoprotein F. Journal of Chromatography B 2016-08-27 [PMID: 27592286]

Bioinformatics

• Gene Symbol: APOF