Recombinant Human Apolipoprotein E/ApoE Protein

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Western Blot: Recombinant Human Apolipoprotein E/ApoE Protein [NBP1-99158] - Lane 1: MW Markers. Lane 2: rh APPO E 3 (1.65ug). Lane 3: rhAPPO E 3 (3.3ug). Lane 4: rh APPO E 3 (6.6 ug).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PAGE
Concentration
Lyoph

Order Details

Recombinant Human Apolipoprotein E/ApoE Protein Summary

Description
Recombinant bioactive protein containing 299 amino acids for Human Apolipoprotein E/ApoE

Source:E. coli
Amino Acid Sequence:(NM_000041)

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
APOE
Purity
>90%, by SDS-PAGE
Endotoxin Note
<0.1 ng/ug

Applications/Dilutions

Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
Sterile filtered and lyophilized from 20 mM Sodium phosphate.
Concentration
Lyoph
Purity
>90%, by SDS-PAGE
Reconstitution Instructions
Reconstitute in dH2O to a concentration of 0.1 - 1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for long-term storage.

Alternate Names for Recombinant Human Apolipoprotein E/ApoE Protein

  • APOE
  • Apo-E
  • Apolipoprotein E
  • apolipoprotein E3
  • late onset)
  • MGC1571

Background

ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solublization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE is an important constituent of all plasma lipoproteins. It's interaction with specific ApoE receptor enables uptake of chylomicron remnants by liver cells, which is an essential step during normal lipid metabolism. ApoE exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution. E3 is the most common isoform and is present in 40-90% of the population. Recombinant human ApoE3 is a 34.0 kDa protein containing 299 amino acid residues. This protein has a N-terminal His-tag.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol APOE