Apolipoprotein E/ApoE Antibody

Images

 
Western Blot: Apolipoprotein E/ApoE Antibody [NB400-158] - Lane 1: rat glial whole cell lysate. Load: 40 ug per lane. Primary antibody: Apolipoprotein E antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

Apolipoprotein E/ApoE Antibody Summary

Immunogen
Full length native apoLipoprotein Type E (purified).
Localization
Secreted
Specificity
Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA.This reacts with human apoLipoprotein E and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, C-III and J apoLipoproteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
APOE
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:4000-1:8000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100
Application Notes
Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.
Positive Control
Apolipoprotein E/ApoE Lysate (NBL1-07621)
Publications
Read Publication using NB400-158.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.125M Sodium Borate, 75mM NaCl and 5mM EDTA, pH 8.0
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Apolipoprotein E/ApoE Antibody

  • APOE
  • Apo-E
  • Apolipoprotein E
  • apolipoprotein E3
  • late onset)
  • MGC1571

Background

At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Apolipoprotein E/ApoE Antibody (NB400-158)(1)

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol APOE
Entrez