Apolipoprotein E/ApoE Antibody

Western Blot: Apolipoprotein E/ApoE Antibody [NB400-158] - Lane 1: rat glial whole cell lysate. Load: 40 ug per lane. Primary antibody: Apolipoprotein E antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:200 for 45 min at RT. Block: 3%BLOTTO overnight at 4C. Predicted/Observed size: 36 kDa, 35-36 kDa for APO-E. MW markers arrows at 25kDa and 37kDa. Other band(s): 55kDa.

Product Discontinued

Apolipoprotein E/ApoE Antibody Summary

• Immunogen: Apolipoprotein E/ApoE was isolated from human plasma by density gradient centrifugation followed by HPLC purification. (Uniprot: P02649)
• Localization: Secreted
• Specificity: Typically less than 1% cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein E and has negligible cross-reactivity with Type A-1 A-II, B, C-I, C-II, C-III and J apoLipoproteins. Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross-reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: APOE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:4000-1:8000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:2000
• Application Notes: This product have been tested by western blot and used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.
• Control:

  Apolipoprotein E/ApoE Overexpression Lysate

Reactivity Notes

This antibody reacts with human apoLipoprotein E and has negligible cross-reactivity with Type A-1 A-II, B, C-I, C-II, C-III and J apoLipoproteins. Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross-reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities

Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Alternate Names for Apolipoprotein E/ApoE Antibody

• APOE
• Apo-E
• Apolipoprotein E
• apolipoprotein E3
• late onset)
• MGC1571

Background

At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apolipoprotein E/ApoE Antibody (NB400-158)(1)

Publication using NB400-158

• Atkinson, K, Blumenstein, M, Black, M et al. An Altered Pattern of Circulating Apolipoprotein E3 Isoforms is Implicated in Preeclampsia. J Lipid Res;50: 71-80. 2009-01-01 [PMID: 18725658]

Bioinformatics

• Gene Symbol: APOE
• Entrez:
  • 348(Human)
• Uniprot:
  • P02649()
  • P02649()
  • P02649()
  • P02649()