Apolipoprotein E/ApoE Antibody

Product Discontinued

Apolipoprotein E/ApoE Antibody Summary

• Immunogen: Full length purified Mouse Apolipoprotein E from pooled mouse plasma high and very low density lipoproteins.
• Localization: Secreted
• Specificity: NB100-240 reacts with mouse ApoE and extremely low recognition of human Apo E. No data available regarding rat activity.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APOE
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunodiffusion
  • Radioimmunodiffusion 1:40
  • Western Blot 1:500-1:1000

Reactivity Notes

Extremely low recognition of human Apo E.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: Neat whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Apolipoprotein E/ApoE Antibody

• APOE
• Apo-E
• Apolipoprotein E
• apolipoprotein E3
• late onset)
• MGC1571

Background

Apolipoprotein E (APOE) is an apolipoprotein located in chylomicrons and intermediate-density lipoproteins that is necessary for the normal catabolism of triglyceride-rich lipoprotein constituents in the liver. APOE has recently been shown to be involseveral biological processes other than lipoprotein transport, including cognition and immunoregulation.

Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.

The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apolipoprotein E/ApoE Antibody (NB100-2040)(2)

Publications using NB100-2040

• Foley K, Hewes A, Garceau D et al. The APOE epsilon 3/epsilon 4 Genotype Drives Distinct Gene Signatures in the Cortex of Young Mice Front Aging Neurosci 2022-04-04 [PMID: 35370604]
• Terwel D, Steffensen KR, Verghese PB et al. Critical Role of Astroglial Apolipoprotein E and Liver X Receptor-{alpha} Expression for Microglial A{beta} Phagocytosis. J Neurosci;31(19):7049-59. 2011-05-11 [PMID: 21562267]

FAQs for Apolipoprotein E/ApoE Antibody (NB100-2040). (Showing 1 - 1 of 1 FAQs).

1. Which part of the APOE protein is recognize by APOE antibody (NB100-2040)? Does this antibody recognize residue 112 and 158?
   • NB100-2040 is a polyclonal antibody and the immunogen is the full length protein. It will bind all along the protein.

Bioinformatics

• Gene Symbol: APOE
• Entrez:
  • 11816(Mouse)