Apolipoprotein B/ApoB Antibody (369717) [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 369717
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

Apolipoprotein B/ApoB Antibody (369717) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human ApoB
  Met1206-Asp1413
  Accession # NP_000375
• Specificity: Detects human Apolipoprotein B in direct ELISAs and Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Apolipoprotein B/ApoB Antibody (369717) [Alexa Fluor® 700]

• Apo B-100
• APOB
• ApoB-100
• ApoB-48
• apolipoprotein B (including Ag(x) antigen)
• Apolipoprotein B
• apolipoprotein B-100
• apolipoprotein B48
• FLDB
• LDLCQ4
• mutant Apo B 100

Background

Human ApoB (Apolipoprotein B-100) is a 550 kDa, secreted, palmitoylated glycoprotein that is part of LDL and VLDL particles. It is made by liver and is 4536 amino acids (aa) in length. It binds LDL to the ApoB/E receptor. Multiple forms of ApoB-100 exist; ApoB-25, 26, 29, 37, 39, 48, and 74. The numbers reflect each molecule’s predicted MW as a percentage of ApoB-100 (512 kDa). Amino acids 1206-1414 of the precursor were used for immunization. Based on likelihood of secretion, this antibody may recognize B26 (1297 aa), B37 (1728 aa), B39 (1799 aa), and B48 (2152 aa). Over the immunization polypeptide range, human ApoB-100 is 80% and 82% aa identical to mouse and dog ApoB-100, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.