APOBEC3G Antibody

Western Blot: APOBEC3G Antibody [NBP1-05026] - Analysis of APOBEC3G in Daudi lysate using NBP1-05026 at 0.5ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: APOBEC3G Antibody [NBP1-05026] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: APOBEC3G Antibody [NBP1-05026] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

APOBEC3G Antibody Summary

• Immunogen: Peptide with sequence C-DEHSQDLSGRLR corresponding to C-Terminus according to NP_068594.1.
• Epitope: C Terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: APOBEC3G
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2-5 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Approx. 45 kDa band observed in Daudi lysates (calculated MW of 46.4 kDa band according to NP_068594.1).
• Control:

  APOBEC3G Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for APOBEC3G Antibody

• APOBEC-related cytidine deaminase
• APOBEC-related protein 9
• APOBEC-related protein
• apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G
• ARCD
• ARP-9
• bK150C2.7
• CEM-15
• CEM15ARP9
• dJ494G10.1
• DNA dC->dU-editing enzyme APOBEC-3G
• EC 3.5.4
• EC 3.5.4.-
• EC 3.5.4.5
• FLJ12740DNA dC->dU editing enzyme
• MDS019
• phorbolin-like protein MDS019

Background

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APOBEC3G Antibody (NBP1-05026)(1)

Publication using NBP1-05026

• Derse D, Hill SA, Princler G et al. Resistance of human T cell leukemia virus type 1 to APOBEC3G restriction is mediated by elements in nucleocapsid. Proc Natl Acad Sci U S A 2007 Feb 13 [PMID: 17299050]

Bioinformatics

• Gene Symbol: APOBEC3G
• Entrez:
  • 60489(Human)
• Uniprot:
  • NP_068594.1(Human)