APOBEC3A Antibody

Product Discontinued

APOBEC3A Antibody Summary

• Immunogen: Peptide with sequence C-TSNFNNGIGRHKTY corresponding to internal region according to NP_663745.1.
• Clonality: Polyclonal
• Host: Goat
• Gene: APOBEC3A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunofluorescence
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1:100 - 1:2000
• Application Notes: WB: Preliminary experiments gave an approx. 28 kDa band in human spleen, tonsil and peripheral blood mononucleocyte lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 23.0 kDa band according to NP_663745.1. The 28 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human tonsil shows staining of distinct parts of the nucleus in lymphoid cells.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for APOBEC3A Antibody

• apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A
• ARP3
• bK150C2.1
• EC 3.5.4
• EC 3.5.4.-
• phorbolin 1
• phorbolin-1
• PHRBNprobable DNA dC->dU-editing enzyme APOBEC-3A

Background

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. This gene encodes a protein that lacks the zinc binding activity and may be an expressed pseudogene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APOBEC3A Antibody (NB100-93428)(4)

Publications using NB100-93428

• Tunou M Generation of KO cell lines of APOBEC 3A, 3B and 3G using CRISPR/Cas9 Brain 2019-11-23 [PMID: 31755958]
• Heller M, Prigge ES, Kaczorowski A et al. APOBEC3A Expression in Penile Squamous Cell Carcinoma Pathobiology. 2017-11-23 [PMID: 29166639] (Human)
• Zahoor MA, Xue G, Sato H et al. HIV-1 Vpr Induces Interferon-Stimulated Genes in Human Monocyte-Derived Macrophages PLoS ONE 2014-08-29 [PMID: 25170834] (WB, Human)
  
  Details:
  Goat anti-APOBEC3A antibody used for western blotting on cell lysates from Ad-Vpr, Ad-Zs or mock-infected MDMs (figure 7).
• Goila-Gaur R, Khan MA, Miyagi E et al. Targeting APOBEC3A to the viral nucleoprotein complex confers antiviral activity. Retrovirology 2007-08-29 [PMID: 17727729]

Bioinformatics

• Gene Symbol: APOBEC3A
• Entrez:
  • 200315(Human)
• Uniprot:
  • P31941(Human)