APIP Antibody (19F461)

Images

 
Western Blot: APIP Antibody (19F461) [NB100-56567] - Analysis of APIP2 in (A) recombinant protein and (B) HeLa cell lysate using APIP antibody at 2 ug/ml.
Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Analysis of human lung cancer tissue section using APIP antibody (clone 19F461) at a concentration of 5 ug/ml. The representative image shows a ...read more
Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Formalin-fixed, paraffin-embedded adenocarcinoma of the breast stained with APIP antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining ...read more
Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Formalin-fixed, paraffin-embedded human stomach stained with APIP antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of ...read more
Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Analysis of formalin-fixed paraffin-embedded human kidney tissue using APIP antibody at 5 ug/ml concentration.
Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Analysis of APIP in a section of normal prostate from human using 5 ug/ml concentration of APIP antibody (clone 19F461). The tubuloalveolar glands in ...read more
Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Detection of APIP in a section of normal human breast tissue using APIP antibody (clone 19F461) at a concentration of 5 ug/ml. The breast's ...read more
Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Analysis of human renal cancer tissue section using APIP antibody (clone 19F461) at a concentration of 5 ug/ml. The representative image shows a ...read more

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, IHC, IHC-P
Clone
19F461
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

APIP Antibody (19F461) Summary

Immunogen
The antibody was developed against a full-length His-tagged recombinant APIP.
Localization
Cytoplasm, Nucleus
Specificity
This monoclonal antibody can be used for detection of APIP and APIP2.
Predicted Species
Bovine (91%). Backed by our 100% Guarantee.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
APIP
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin
  • Western Blot 1-2 ug/ml
Control
APIP Overexpression Lysate
Publications
Read Publications using
NB100-56567 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (94%), Bovine (91%) Use in Mouse reported in scientific literature (PMID:31263105).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for APIP Antibody (19F461)

  • APAF1 interacting protein
  • APAF1-interacting protein
  • APIP2
  • CGI29
  • CGI-29
  • dJ179L10.2
  • EC 4.2.1.109
  • Mmrp19
  • MTRu-1-P dehydratase
  • probable methylthioribulose-1-phosphate dehydratase

Background

The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis3. Recently, Cho et al have identified a new Apaf-1 Interactiong Protein (APIP) also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf1. APIP is predicted to code for a 204 amino acid. An isoform of APIP, APIP2 encodes a 242 amino acid protein, which is an alternative splicing variant differing in its N-terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for APIP Antibody (NB100-56567)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC, WB.


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IHC
(1)
WB
(1)
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Filter By Species
Mouse
(2)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol APIP
Entrez
Uniprot