APIP Antibody (19F461)

Western Blot: APIP Antibody (19F461) [NB100-56567] - Analysis of APIP2 in (A) recombinant protein and (B) HeLa cell lysate using APIP antibody at 2 ug/ml.

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Analysis of human lung cancer tissue section using APIP antibody (clone 19F461) at a concentration of 5 ug/ml. The representative image shows a nuclear and cytoplasmic staining pattern of APIP expression in lung cancer cells.

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Formalin-fixed, paraffin-embedded adenocarcinoma of the breast stained with APIP antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Formalin-fixed, paraffin-embedded human stomach stained with APIP antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Analysis of formalin-fixed paraffin-embedded human kidney tissue using APIP antibody at 5 ug/ml concentration.

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Analysis of APIP in a section of normal prostate from human using 5 ug/ml concentration of APIP antibody (clone 19F461). The tubuloalveolar glands in prostate section depicted APIP positivity in the cytoplasm and nuclei of the epithelial cells. The fibro-muscular stroma showed an overall weak staining with intense nuclear positivity in some cells.

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Detection of APIP in a section of normal human breast tissue using APIP antibody (clone 19F461) at a concentration of 5 ug/ml. The breast's ductal/acinar epithelial cells showed strong cytoplasmic as well as nuclear expression, whereas the myoepithelial cells and the intra-lobular connective tissue depicted very weak to negligible APIP positivity.

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Analysis of human renal cancer tissue section using APIP antibody (clone 19F461) at a concentration of 5 ug/ml. The representative image shows a nuclear and cytoplasmic staining pattern of APIP expression in cancer cells.

• Reactivity: Hu, Mu, Bv
• Applications: WB, IHC
• Clone: 19F461
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

APIP Antibody (19F461) Summary

• Immunogen: The antibody was developed against a full-length His-tagged recombinant APIP.
• Localization: Cytoplasm, Nucleus
• Specificity: This monoclonal antibody can be used for detection of APIP and APIP2.
• Predicted Species: Bovine (91%). Backed by our 100% Guarantee.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: APIP
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin
  • Western Blot 1-2 ug/ml
• Control:

  APIP Overexpression Lysate

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (91%) Use in Mouse reported in scientific literature (PMID:31263105).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for APIP Antibody (19F461)

• APAF1 interacting protein
• APAF1-interacting protein
• APIP2
• CGI29
• CGI-29
• dJ179L10.2
• EC 4.2.1.109
• Mmrp19
• MTRu-1-P dehydratase
• probable methylthioribulose-1-phosphate dehydratase

Background

The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis3. Recently, Cho et al have identified a new Apaf-1 Interactiong Protein (APIP) also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf1. APIP is predicted to code for a 204 amino acid. An isoform of APIP, APIP2 encodes a 242 amino acid protein, which is an alternative splicing variant differing in its N-terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APIP Antibody (NB100-56567)(2)

Publications using NB100-56567

• Mowry FE, Peaden SC, Stern JE, Biancardi VC TLR4 and AT1R mediate blood-brain barrier disruption, neuroinflammation, and autonomic dysfunction in spontaneously hypertensive rats Pharmacological research 2021-10-02 [PMID: 34610452] (IF/IHC, Mouse)
• Lim B, Jung K et al. Cardioprotective role of APIP in myocardial infarction through ADORA2B. Cell Death Dis 2019-01-07 [PMID: 31263105] (WB, Mouse)

Bioinformatics

• Gene Symbol: APIP
• Entrez:
  • 508345(Bovine)
  • 51074(Human)
  • 56369(Mouse)
• Uniprot:
  • Q96GX9(Human)