APH1A Antibody

Images

 
Western Blot: APH1A Antibody [NBP1-77272] - Analysis of APH1 in RAW264.7 cell lysate with APH1 antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry-Paraffin: APH1A Antibody [NBP1-77272] - Human brain tissue with APH1a antibody at 5 ug/ml.
Western Blot: APH1A Antibody [NBP1-77272] - Human brain tissue lysate with APH1a antibody at (A) 0.5 and (B) 1 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

APH1A Antibody Summary

Immunogen
Antibody was raised against a 18 amino acid synthetic peptide from near the center of human APH1. The immunogen is located within amino acids 80 - 130 of APH1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
APH1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
APH1A Peptide (NBP1-77272PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for APH1A Antibody

  • 6530402N02Rik
  • anterior pharynx defective 1 homolog A (C. elegans)
  • APH-1
  • APH1A
  • APH-1A
  • aph-1alpha
  • CGI-78
  • gamma-secretase subunit APH-1A
  • Presenilin-stabilization factor
  • PSF

Background

APH1 was initially identified as a component of the Notch pathway in C. elegans. Along with nicastrin, PEN2, and presenilin-1 APH1 is an essential component of the gamma-secretase complex which cleave the amyloid precursor protein (APP) at what are known as the gamma- and epsilon-sites and can lead to the accumulation of the Amyloid beta peptide (Abeta) cleavage product that is associated with Alzheimer's disease. APH1 exists in at least three distinct isoforms with APH1a as the principal isoform present in the gamma-secretase complex. Mice deficient in this isoform, but not the other two, were lethal at E10.5, with impaired vascular and neural development observed. Despite its predicted molecular weight, APH1 protein often migrates at aberrant locations in SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol APH1A
Entrez
Uniprot