AP4B1 Overexpression Lysate

Images

 
Western Blot: AP4B1 Overexpression Lysate (Adult Normal) [NBL1-07588] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AP4B1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

AP4B1 Overexpression Lysate Summary

Description

AP4B1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC204338

Accession#: NM_006594

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
AP4B1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
83.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for AP4B1 Overexpression Lysate

  • Adapter-related protein complex 4 subunit beta-1
  • adaptor-related protein complex 4, beta 1 subunit
  • AP-4 adapter complex subunit beta
  • AP-4 complex subunit beta-1
  • beta 4 subunit of AP-4
  • Beta subunit of AP-4
  • BETA-4
  • beta4-adaptin

Background

AP4B1, or AP-4 complex subunit beta-1, consists of a 739 amino acid isoform that is 83 kDa, and is involved in targeting specific proteins that move between the trans-Golgi network and the endosomal-lysosomal system. The AP4B1 protein is included in current research on a variety of diseases and disorders including cerebral palsy, neuronitis, malaria, spasticity, spastic paraplegia, hypotonia, intellectual disability, and cerebritis. The protein interacts with AP4E1, MY, AP4M1, BUB1, and BUB1B in several biological processes, such as membrane trafficking, Golgi associated vesicle biogenesis, and clathrin derived vesicle budding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for AP4B1 Lysate (NBL1-07588) (0)

There are no publications for AP4B1 Lysate (NBL1-07588).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for AP4B1 Lysate (NBL1-07588) (0)

There are no reviews for AP4B1 Lysate (NBL1-07588). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for AP4B1 Lysate (NBL1-07588) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional AP4B1 Products

Blogs on AP4B1

There are no specific blogs for AP4B1, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our AP4B1 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol AP4B1