Ankyrin 3 Antibody

Western Blot: Ankyrin 3 Antibody [NBP3-05549] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody at a dilution of 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction. Bands at 60-190kDa represent Ankyrin 3 splice variants and proteolytic fragments. Larger Ankyrin 3 isoforms of 270kDa and 480kDa can be seen on longer exposure of the blots.

Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05549] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Ankyrin 3 Antibody, CPCA-ANK3, at a dilution of 1:2,000 in green, and costained with mouse mAb to RBFOX3/NeuN at a dilution of 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The Ankyrin 3 antibody stains the axonal initial segments, while the RBFOX3/NeuN antibody reveals perikarya and nuclei of neurons.

• Reactivity: Hu, Mu, Rt, Bv, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Ankyrin 3 Antibody Summary

• Immunogen: The C-terminal 398 amino acids of human Ankyrin 3 expressed in and purified from E. coli.
• Clonality: Polyclonal
• Host: Chicken
• Gene: ANK3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:500-1:1000
  • Western Blot 1:5000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol (1:1)
• Preservative: 5mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for Ankyrin 3 Antibody

• ankyrin G
• Ankyrin G119
• ankyrin-3
• ankyrin-G
• Node Of Ranvier

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANK3