Ankyrin 3 Antibody (S106-20) - BSA Free

Western Blot: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Western Blot analysis of Rat brain membrane lysate showing detection of Ankyrin 3 protein using Mouse Anti-Ankyrin 3 Monoclonal Antibody, Clone S106-20 (NBP2-59310). Primary Antibody: Mouse Anti-Ankyrin 3 Monoclonal Antibody (NBP2-59310) at 1:1000.

Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin 3 Monoclonal Antibody, Clone S106-20 (NBP2-59310). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin 3 Monoclonal Antibody (NBP2-59310) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin 3 Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin G Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X.

Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin 3 Monoclonal Antibody, Clone S106-20 (NBP2-59310). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin 3 Monoclonal Antibody (NBP2-59310) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Ankyrin 3 Antibody. (D) Composite.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: S106-20
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Ankyrin 3 Antibody (S106-20) - BSA Free Summary

• Immunogen: Fusion protein 1000 C-terminal amino acids of human Ankyrin 3 (also known as ANK-3 or Ankyrin G) encompassing all of Ankyrin 3 with the exception of Ankyrin repeats.
• Localization: Cytoplasm , Cytoskeleton , Cell Projection , Axon , Cell Membrane , Sarcolemma , Cell Junction , Synapse , Postsynaptic Cell Membrane , Lysosome
• Specificity: Detects >200kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANK3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for Ankyrin 3 Antibody (S106-20) - BSA Free

• ankyrin G
• Ankyrin G119
• ankyrin-3
• ankyrin-G
• Node Of Ranvier

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ankyrin 3 Antibody (NBP2-59310)(2)

Publications using NBP2-59310

• Radler MR, Liu X, Peng M et al. Pyramidal neuron morphogenesis requires a septin network that stabilizes filopodia and suppresses lamellipodia during neurite initiation Current biology : CB 2022-12-12 [PMID: 36538929]
• Devlin L Proteomic Profiling of the Oncogenic Septin 9 Reveals Isoform-Specific Interactions and Functions in Breast Cancer Cells Drexel University 2022-03-03 [PMID: 34409731]

Bioinformatics

• Gene Symbol: ANK3