Ankyrin 1 Antibody

Product Discontinued

Ankyrin 1 Antibody Summary

• Immunogen: Peptide with sequence C-QIVKRASLKRGKQ corresponding to C-Terminus according to NP_065208.2, NP_065209.2, NP_065210.2, NP_065211.2, NP_065213.2.
• Epitope: C Terminus
• Specificity: This antibody is expected to recognise isoform 3 (NP_000028.3) and isoform 5 (NP_065211.2).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ANK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
• Application Notes: WB: Preliminary experiments gave approx. 19-23 kDa band bands in human skeletal muscle lysates after 0.01 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the observed 28+30 kDa band bands in the literature for this tissuescientific literature (PMID: 9430667) and given the calculated size of 17.6 kDa band according to NP_065211.2. The 19-22 kDa band bands were successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Ankyrin 1 Antibody

• ANK
• ANK-1
• ankyrin 1, erythrocytic
• ankyrin-1
• ankyrin-R
• Erythrocyte ankyrin
• SPH1
• SPH2

Background

Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actincytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and themaintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various targetproteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed ofthree structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highlyconserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subjectto variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has alsobeen found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of allpatients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving riseto different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting fromusage of an alternate promoter have also been identified. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ankyrin 1 Antibody (NBP1-02311)(1)

Publication using NBP1-02311

• Dubreuil RR. Functional links between membrane transport and the spectrin cytoskeleton. J Membr Biol 2006-01-01 [PMID: 17091212]

Bioinformatics

• Gene Symbol: ANK1
• Entrez:
  • 286(Human)
• Uniprot:
  • NP_000028.3(Human)