ANAPC2 Antibody (OTI1A6) [Janelia Fluor® 549]

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI1A6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 549

ANAPC2 Antibody (OTI1A6) [Janelia Fluor® 549] Summary

• Immunogen: Full length recombinant protein of human ANAPC2 (NP_037498) produced in HEk293 cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANAPC2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for ANAPC2 Antibody (OTI1A6) [Janelia Fluor® 549]

• anaphase promoting complex subunit 2
• anaphase-promoting complex subunit 2
• APC2KIAA1406Cyclosome subunit 2
• RP11-350O14.5

Background

ANAPC2 is a large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANAPC2