AMOT Antibody

Western Blot: AMOT Antibody [H00154796-B01P] - Analysis of AMOT expression in transfected 293T cell line by AMOT polyclonal antibody. Lane 1: AMOT transfected lysate(74.25 KDa). Lane 2: Non-transfected lysate.

Western Blot: AMOT Antibody [H00154796-B01P] - analysis of AMOT expression in transfected 293T cell line by AMOT purified MaxPab polyclonal antibody (B01P). Lane 1: AMOT transfected lysate (72.50 KDa). Lane 2: Non-transfected lysate.

Western Blot: AMOT Antibody [H00154796-B01P] - AMOT MaxPab polyclonal antibody. Western Blot analysis of AMOT expression in rat brain.

Product Discontinued

AMOT Antibody Summary

• Immunogen: AMOT (NP_573572.1, 1 a.a. - 675 a.a.) full-length human protein. MPRAQPSSASYQPVPADPFAIVSRAQQMVEILSDENRNLRQELEGCYEKVARLQKVETEIQRVSEAYENLVKSSSKREALEKAMRNKLEGEIRRMHDFNRDLRERLETANKQLAEKEYEGSEDTRKTISQLFAKNKESQREKEKLEAELATARSTNEDQRRHIEIRDQALSNAQAKVVKLEEELKKKQVYVDKVEKMQQALVQLQAACEKREQLEHRLRTRLERELESLRIQQRQGNCQPTNVSEYNAAALMELLREKEERILALEADMTKWEQKYLEENVMRHFALDAAATVAAQRDTTVISHSPNTSYDTALEARIQKEEEEILMANKRCLDMEGRIKTLHAQIIEKDAMIKVLQQRSRKEPSKTEQLSCMRPAKSLMSISNAGSGLLSHSSTLTGSPIMEEKRDDKSWKGSLGILLGGDYRAEYVPSTPSPVPPSTPLLSAHSKTGSRDCSTQTERGTESNKTAAVAPISVPAPVAAAATAAAITATAATITTTMVAAAPVAVAAAAAPAAAAAPSPATAAATAAAVSPAAAGQIPAAASVASAAAVAPSAAAAAAVQVAPAAPAPVPAPALVPVPAPAAAQASAPAQTQAPTSAPAVAPTPAPTPTPAVAQAEVPASPATGPGPHRLSIPSLTCNPDKTDGPVFHSNTLERKTPIQILGQEPDAEMVEYLI
• Specificity: AMOT - angiomotin,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: AMOT
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and tissue lysate for Western Blot. Has also been used for ELISA. Use in In vitro reported in scientific literature (PMID: 27321186).

Reactivity Notes

Rat determined by Western Blot.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AMOT Antibody

• angiomotin p80 isoform
• angiomotin
• KIAA1071angiomotin p130 isoform

Background

This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AMOT Antibody (H00154796-B01P)(4)

Publications using H00154796-B01P

• Nguyen HT, Andrejeva D, Gupta R et al. Deubiquitylating enzyme USP9x regulates hippo pathway activity by controlling angiomotin protein turnover. Cell Discov 2016-03-29 [PMID: 27462448]
• Wang Y, Justilien V, Brennan KI et al. PKCL regulates nuclear YAP1 localization and ovarian cancer tumorigenesis. Oncogene 2016-06-20 [PMID: 27321186] (In vitro)
• Chan SW, Lim CJ, Guo F et al. Actin-binding and Cell Proliferation Activities of Angiomotin Family Members are Regulated by Hippo Pathway-mediated Phosphorylation. J Biol Chem. 2013-11-13 [PMID: 24225952]
• Chan SW, Lim CJ, Chong YF et al. Hippo Pathway-independent Restriction of TAZ and YAP by Angiomotin. J Biol Chem. 2011-01-11 [PMID: 21224387]

Bioinformatics

• Gene Symbol: AMOT
• Entrez:
  • 154796(Human)
• Uniprot:
  • NP_573572.1(Human)