PLEKHG5 Antibody (5A9) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: PLEKHG5 Antibody (5A9) [H00057449-M01] - Analysis of monoclonal antibody to PLEKHG5 on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: PLEKHG5 Antibody (5A9) [H00057449-M01] - Analysis of monoclonal antibody to PLEKHG5 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1.5 ug/ml.

ELISA: PLEKHG5 Antibody (5A9) [H00057449-M01] - Detection limit for recombinant GST tagged PLEKHG5 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 5A9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PLEKHG5 Antibody (5A9) - Azide and BSA Free Summary

• Immunogen: PLEKHG5 (NP_065682, 896 a.a. ~ 995 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SAPSRSLSELCLAVPAPGIRTQGSPQEAGPSWDCRGAPSPGSGPGLVGCLAGEPAGSHRKRCGDLPSGASPRVQPEPPPGVSAQHRKLTLAQLYRIRTTL
• Specificity: PLEKHG5 (5A9)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLEKHG5
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PLEKHG5 Antibody (5A9) - Azide and BSA Free

• CMTRIC
• DSMA4
• GEF720
• KIAA0720Guanine nucleotide exchange factor 720
• NFkB activating protein
• novel PH domain-containing protein
• PH domain-containing family G member 5
• pleckstrin homology domain containing family G member 5
• pleckstrin homology domain containing, family G (with RhoGef domain) member 5
• pleckstrin homology domain-containing family G member 5
• Syx
• Tech

Background

This gene encodes a protein that activates the nuclear factor kappa B (NFKB1) signaling pathway. Multations in this gene have been found in a family with distal spinal muscular atrophy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLEKHG5 Antibody (H00057449-M01)(5)

Publications using H00057449-M01

• Kaya W, Carsten S, Melanie L et al. PLEKHG5 regulates autophagy, survival and MGMT expression in U251-MG glioblastoma cells. Sci Rep. 2020-12-14 [PMID: 33318498]
• Hetmanski JHR, de Belly H, Busnelli I et al. Membrane Tension Orchestrates Rear Retraction in Matrix-Directed Cell Migration Dev. Cell 2019-10-09 [PMID: 31607653]
• Dachsel JC, Ngok SP, Lewis-Tuffin LJ et al. The RhoGEF Syx regulates the balance of Dia and ROCK activities to promote polarized cancer cell migration. Mol Cell Biol. 2013-10-14 [PMID: 24126053] (WB, Human)
• Ngok SP, Geyer R, Kourtidis A et al. Phosphorylation-mediated 14-3-3 Protein Binding Regulates the Function of the Rho-specific Guanine Nucleotide Exchange Factor (RhoGEF) Syx. J Biol Chem. 2013-01-18 [PMID: 23335514]
• Ngok SP, Geyer R, Liu M et al. VEGF and Angiopoietin-1 exert opposing effects on cell junctions by regulating the Rho GEF Syx. J Cell Biol. 2012-12-17 [PMID: 23253477]

Bioinformatics

• Gene Symbol: PLEKHG5
• Entrez:
  • 57449(Human)
• Uniprot:
  • NP_065682(Human)