AMFR/gp78 Antibody

Western Blot: AMFR/gp78 Antibody [NBP2-15374] - Sample (30 ug of whole cell lysate) A: K562 7. 5% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: AMFR/gp78 Antibody [NBP2-15374] - Cal27 xenograft. AMFR antibody [N3C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: AMFR/gp78 Antibody [NBP2-15374] - Human hepatoma, using AMFR antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Discontinued

AMFR/gp78 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human AMFR. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum membrane
• Predicted Species: Mouse (95%), Bovine (95%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AMFR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Functional
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • In vitro assay
  • Western Blot 1:500-1:3000
• Application Notes: Functional and In Vitro usages reported in scientific literature (PMID: 25945836). Please note this product contains Thimerosal, BSA, and Glycerol. You may need to perform a buffer exchange before using this product with live cells.
• Theoretical MW: 73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for AMFR/gp78 Antibody

• AMF receptor
• AMF receptor, isoform 1
• AMF receptor, isoform 2
• AMFR
• autocrine motility factor receptor
• Autocrine motility factor receptor, isoform 2
• gp78
• RING finger protein 45
• RNF45
• RNF45EC 6.3.2.-

Background

Autocrine motility factor is a tumor motility-stimulating protein secreted by tumor cells. The protein encoded by this gene is a glycosylated transmembrane protein and a receptor for autocrine motility factor. The receptor, which shows some sequence similarity to tumor protein p53, is localized to the leading and trailing edges of carcinoma cells. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for AMFR/gp78 Antibody (NBP2-15374)(3)

Publications using NBP2-15374

• McKenna MJ, Adams BM, Chu V et al. ATP13A1 prevents ERAD of folding-competent mislocalized and misoriented proteins Molecular cell 2022-10-15 [PMID: 36283413]
• Lucarelli G, Rutigliano M, Sanguedolce F et al. Increased Expression of the Autocrine Motility Factor is Associated With Poor Prognosis in Patients With Clear Cell-Renal Cell Carcinoma. Medicine (Baltimore). 2015-11-01 [PMID: 26579829] (IHC-P, Human)
• Lucarelli G, Galleggiante V, Rutigliano M et al. Metabolomic profile of glycolysis and the pentose phosphate pathway identifies the central role of glucose-6-phosphate dehydrogenase in clear cell-renal cell carcinoma. Oncotarget. [PMID: 25945836] (In vitro, IHC-P, Func, Human)
  
  Details:
  Citation using the FITC form of this antibody.

Bioinformatics

• Gene Symbol: AMFR
• Uniprot:
  • Q9UKV5(Human)