AMFR/gp78 Antibody

Images

 
Western Blot: AMFR Antibody [NBP2-15374] - Sample (30 ug of whole cell lysate) A: K562 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: AMFR Antibody [NBP2-15374] - Cal27 xenograft.
Immunohistochemistry-Paraffin: AMFR Antibody [NBP2-15374] - Paraffin-embedded Hepatoma, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Func, IHC, IHC-P, In vitro
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

AMFR/gp78 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human AMFR. The exact sequence is proprietary.
Localization
Endoplasmic reticulum membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AMFR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Functional
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • In vitro assay
Application Notes
Functional and In Vitro usages reported in scientific literature (PMID: 25945836).
Theoretical MW
73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-15374 in the following applications:

Reactivity Notes

Expected cross reactivity based on sequence homology: Chimpanzee (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AMFR/gp78 Antibody

  • AMF receptor
  • AMF receptor, isoform 1
  • AMF receptor, isoform 2
  • AMFR
  • autocrine motility factor receptor
  • Autocrine motility factor receptor, isoform 2
  • gp78
  • RING finger protein 45
  • RNF45
  • RNF45EC 6.3.2.-

Background

Autocrine motility factor is a tumor motility-stimulating protein secreted by tumor cells. The protein encoded by this gene is a glycosylated transmembrane protein and a receptor for autocrine motility factor. The receptor, which shows some sequence similarity to tumor protein p53, is localized to the leading and trailing edges of carcinoma cells. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-02541
Species: Hu, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NB120-11433
Species: Hu, Mu, Rt, Ye
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-89150
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
H00011026-M01
Species: Hu
Applications: WB, ELISA
NBP1-31302
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-2526
Species: Hu, Mu
Applications: WB, ICC/IF, IHC-P, KO
NBP2-46278
Species: Hu
Applications: WB, IHC, IF
NB110-40591
Species: Hu
Applications: WB, IHC-P, IP
NBP1-88680
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-00990
Species: Hu, Mu, Rt, Ca, Mk, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-85310
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-21037
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-59068
Species: Hu
Applications: WB
NBP1-81988
Species: Hu
Applications: IHC, IHC-P
NB100-77316
Species: Hu, Mu
Applications: WB (-), ICC/IF, IHC, IHC-P, IP
NBP1-91996
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NB120-11433
Species: Hu, Mu, Rt, Ye
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-15374
Species: Hu
Applications: WB, Func, IHC, IHC-P, In vitro

Publications for AMFR/gp78 Antibody (NBP2-15374)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Func, IHC-P, in vitro.


Filter By Application
Func
(1)
IHC-P
(2)
in vitro
(1)
All Applications
Filter By Species
Human
(2)
All Species
Showing Publications 1 - 2 of 2.
Publications using NBP2-15374 Applications Species
Lucarelli G, Rutigliano M, Sanguedolce F et al. Increased Expression of the Autocrine Motility Factor is Associated With Poor Prognosis in Patients With Clear Cell-Renal Cell Carcinoma. Medicine (Baltimore). 2015 Nov 01 [PMID: 26579829] (IHC-P, Human) IHC-P Human
Lucarelli G, Galleggiante V, Rutigliano M et al. Metabolomic profile of glycolysis and the pentose phosphate pathway identifies the central role of glucose-6-phosphate dehydrogenase in clear cell-renal cell carcinoma. Oncotarget. 2015 Apr 14 [PMID: 25945836] (IHC-P, Func, in vitro, Human)

Details:
AMFR/gp78 antibody was used for IHC-P on human primary renal tumors and non neoplastic tissues collected from patients who underwent radical or partial nephrectomy for clear cell-renal cell carcinoma/ccRCC. The assay involved 5um paraffin-embedded tissue sections, microwave based antigen retrieval in citrate buffer 0.01M pH 6.0, use of primary antibody at 1: 100 dilution with 2 hours RT incubation, detection using Real EnVision Detection System -Peroxidase/DAB kit (Figure 4B). The antibody was used in double-staining assays along with Glucose 6 phosphate isomerase/G6PI using Immunofluorescence based detection (Figure 4C). The antibody was used for in vitro functional application also in the wound healing assay wherein the cultured cells were treated with 2ng/ul of antibody for 30 minutes (Figure 5). See full text for detailed protocl and staining images.
IHC-P, Func, in vitro Human

Reviews for AMFR/gp78 Antibody (NBP2-15374) (0)

There are no reviews for AMFR/gp78 Antibody (NBP2-15374). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for AMFR/gp78 Antibody (NBP2-15374) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional AMFR/gp78 Products

Bioinformatics Tool for AMFR/gp78 Antibody (NBP2-15374)

Discover related pathways, diseases and genes to AMFR/gp78 Antibody (NBP2-15374). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for AMFR/gp78 Antibody (NBP2-15374)

Discover more about diseases related to AMFR/gp78 Antibody (NBP2-15374).
 

Pathways for AMFR/gp78 Antibody (NBP2-15374)

View related products by pathway.

PTMs for AMFR/gp78 Antibody (NBP2-15374)

Learn more about PTMs related to AMFR/gp78 Antibody (NBP2-15374).
 

Research Areas for AMFR/gp78 Antibody (NBP2-15374)

Find related products by research area.

Blogs on AMFR/gp78

There are no specific blogs for AMFR/gp78, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our AMFR/gp78 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol AMFR
Entrez
Uniprot