Aly Antibody (11G5)

Images

 
Western Blot: Aly Antibody (11G5) [NB100-670] - La cell extract was separated by SDS-PAGE and probed with Monoclonal Anti-ALY (0.4 ug/mL, lane 1) followed by Goat Anti-Mouse, Peroxidase conjugate (Cat. No. A2304, lane ...read more

Product Details

Summary
Reactivity Hu, XpSpecies Glossary
Applications WB, ELISA, ICC/IF, IP, MiAr
Clone
11G5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
2.1 mg/ml

Order Details

Aly Antibody (11G5) Summary

Immunogen
Recombinant human ALY / REF protein
Localization
Nuclear
Specificity
This is specific for Aly protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ALYREF
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Microarray
Application Notes
Co-immunoprecipitation and western blot studies were performed in Hela cells using a monoclonal mouse anti-Aly antibody. The results indicated that Aly which was observed as a 30 kDa band interacted with the Herpes Simplex Virus I protein, ICP27. 1
Positive Control
HeLa Lysate (NB800-PC1)
Aly Lysate (NBL1-16889)
Publications
Read Publications using NB100-670.

Reactivity Notes

Cross-reacts with Human and Xenopus laevis. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
2.1 mg/ml
Purity
Protein A or G purified

Alternate Names for Aly Antibody (11G5)

  • Ally of AML-1 and LEF-1
  • ALY/REF
  • ALYBEFbZIP enhancing factor
  • bZIP-enhancing factor BEF
  • REF
  • THO complex 4
  • THO complex subunit 4
  • tho4
  • Transcriptional coactivator Aly/REF

Background

Pre-mRNA splicing plays an important role in regulation of gene expression. During the splicing process, specific proteins are recruited to the mRNA and assembled into a complex of mRNA-protein near the exon-exon junction. This complex is termed the mRNA-protein complex (mRNP) or the exon-exon junction complex. The proteins that are found in this complex include: Y14, magoh, Aly/REF, RNPS1, Upf3, and DEK.1-5 Aly (also known as mREF1-I) is the mammalian homologue of the yeast mRNA export factor Yra1p.6-7 It is a 233 amino acid RNAbinding protein that contains a RNA-binding domain and is recruited to the mRNP complexes during the splicing. Excess of recombinant expression of Aly in the cells increases both the rate and efficiency of spliced and unspliced mRNA export (in vivo) from the nucleus to the cytoplasm. In contrast to the Y14 protein, Aly is dissociated from the mRNAs after the export to the cytoplasm.1, 5-6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aly Antibody (NB100-670)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ALYREF
Entrez
Uniprot