AlphaB Crystallin/CRYAB Antibody

Product Discontinued

AlphaB Crystallin/CRYAB Antibody Summary

• Immunogen: A synthetic peptide made to a C-terminal region of the human Crystallin AB protein (within residues 100-175). [Swiss-Prot P02511]
• Predicted Species: Mouse (92%), Rat (100%), Porcine (100%), Bovine (100%), Rabbit (100%), Hamster (100%), Sheep (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRYAB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1: 50 - 1:150
  • Western Blot 0.5-1 ug/ml
• Application Notes: This Crystallin AB antibody is useful for Western blot analysis, where a band is seen at ~20 kDa. There is also a band of greater intensity at ~40 kDa that may be an aggregated heteromeric complex. **CryAB aggregates with homologous proteins, including CryAA and the small heat shock protein HSP28, to form large heteromeric complexes. Use in ELISA, Flow Cytometry reported in scientific literature (PMID: 23586607).
• Control:

  Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
  HSP27 Overexpression Lysate
  Mouse Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Predicted to react with rat, sheep, rabbit, monkey, hamster, cow, and pig based on 100% sequence homology. Immunogen sequence has 92% homology to mouse. Mouse reactivity reported in scientific literature (PMID: 23586607).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.1% Sodium Azide
• Concentration: 0.9 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AlphaB Crystallin/CRYAB Antibody

• alpha B crystallin
• alpha(B)-crystallin
• AlphaB Crystallin
• alpha-crystallin B chain
• CRYA2
• CRYA2alpha crystallin B chain
• CRYAB
• crystallin, alpha B
• CTPP2
• Heat shock protein beta-5
• heat-shock 20 kD like-protein
• HSPB5
• Renal carcinoma antigen NY-REN-27
• Rosenthal fiber component

Background

Crystallin AB (CRYAB or alpha-basic-crystallin) belongs to HSP20 family and was originally discovered as chaperone abundantly expressed in eye lens where it plays a key role in maintaining transparency and refractive index of lens. Subsequently, CRYAB was found to acts as a molecular chaperone induced by a variety of stress stimuli, conferring cytoprotection by suppressing aggregation of denatured protein. In its anti-apoptotic role, CRYAB protects cells against stress, TNF-alpha, apoptotic effectors etc. and can inhibit caspase-3/PARP alongwith preventing Bax/Bcl-2 translocation. CRYAB is a heteropolymer of three CRYAA and one CRYAB subunits, and can aggregate with homologous proteins (e.g. HSPB1) to form large complexes and interact with HSPBAP1 as well as TTN/titin. Localized in cytoplasm, during heat shock stimulus, CRYAB translocates to sub-nuclear structures known as SC35/nuclear splicing speckles. CRYAB is expressed in several other tissues such as muscle, brain, spleen, lung, kidney, skin etc and has been shown to implicate in certain diseases including cardiac myopathies, neurodegenerative diseases and malignancies including brain, breast, prostate and renal cancers. Defectsive CRYAB causes myopathy myofibrillar type 2 (MFM2), cataract posterior polar type 2 (CTPP2) and myopathy myofibrillar fatal infantile hypertonic alpha-B crystallin-related (MFMFIH-CRYAB).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AlphaB Crystallin/CRYAB Antibody (NB100-2520)(2)

Publications using NB100-2520

• Chauhan VS, Nelson DA, Marriott I, Bost KL. Alpha beta-crystallin expression and presentation following infection with murine gammaherpesvirus 68. Autoimmunit 2013-04-16 [PMID: 23586607] (FACS, ELISA, Mouse)
  
  Details:
  Using the Biotin conjugated version of NB100-2520, catalog number NB100-2520B.
• Wu, G, Glickstein, S, Liu, W, Fujita, T, Li, W, Yang, Q, Duvoisin, R, Wan, Y. The Anaphase-promoting Complex Coordinates Initiation of Lens Differentiation, . 18, 1018-1029. 2007-01-01 [PMID: 17215516]

FAQs for AlphaB Crystallin/CRYAB Antibody (NB100-2520). (Showing 1 - 1 of 1 FAQs).

1. I expect it to be proprietary, but would it be possible to check if position 78 is definitely not part of the immunogen? I know that on the webpage the immunogen is mentioned to be aa 100-175.
   • The immunogen for NB100-2520 lies within aa 100-175. I can confirm that it does not include aa 78.

Bioinformatics

• Gene Symbol: CRYAB