AlphaB Crystallin/CRYAB Antibody


Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2520] - Analysis of Lane 1: human skeletal muscle and Lane 2: mouse skeletal muscle proteins using NB100-2520.
Immunocytochemistry/ Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB100-2520] - CRYAB antibody was tested in SHSH5Y cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) more

Product Details

Reactivity HuSpecies Glossary
Applications WB, ICC/IF
0.9 mg/ml

AlphaB Crystallin/CRYAB Antibody Summary

A synthetic peptide made to a C-terminal region of the human Crystallin AB protein (within residues 100-175). [Swiss-Prot P02511]
Predicted Species
Mouse (92%), Rat (100%), Porcine (100%), Rabbit (100%), Primate (100%), Sheep (100%), Hamster (100%), Bovine (100%)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-citrate/phosphate, pH 7-8
0.1% Sodium Azide
0.9 mg/ml
Immunogen affinity purified


Application Notes
This Crystallin AB antibody is useful for Western blot analysis, where a band is seen at ~20 kDa. There is also a band of greater intensity at ~40 kDa that may be an aggregated heteromeric complex. **CryAB aggregates with homologous proteins, including CryAA and the small heat shock protein HSP28, to form large heteromeric complexes.
Control Peptide
AlphaB Crystallin/CRYAB Peptide (NB100-2520PEP)
Read Publications using
NB100-2520 in the following applications:

Reactivity Notes

Predicted to react with rat, sheep, rabbit, monkey, hamster, cow, and pig based on 100% sequence homology. Immunogen sequence has 92% homology to mouse.

Alternate Names for AlphaB Crystallin/CRYAB Antibody

  • alpha B crystallin
  • alpha(B)-crystallin
  • alpha-crystallin B chain
  • CRYA2
  • CRYA2alpha crystallin B chain
  • crystallin, alpha B
  • CTPP2
  • Heat shock protein beta-5
  • heat-shock 20 kD like-protein
  • HSPB5
  • Renal carcinoma antigen NY-REN-27
  • Rosenthal fiber component


Crystallin AB (CRYAB or alpha-basic-crystallin) belongs to HSP20 family and was originally discovered as chaperone abundantly expressed in eye lens where it plays a key role in maintaining transparency and refractive index of lens. Subsequently, CRYAB was found to acts as a molecular chaperone induced by a variety of stress stimuli, conferring cytoprotection by suppressing aggregation of denatured protein. In its anti-apoptotic role, CRYAB protects cells against stress, TNF-alpha, apoptotic effectors etc. and can inhibit caspase-3/PARP alongwith preventing Bax/Bcl-2 translocation. CRYAB is a heteropolymer of three CRYAA and one CRYAB subunits, and can aggregate with homologous proteins (e.g. HSPB1) to form large complexes and interact with HSPBAP1 as well as TTN/titin. Localized in cytoplasm, during heat shock stimulus, CRYAB translocates to sub-nuclear structures known as SC35/nuclear splicing speckles. CRYAB is expressed in several other tissues such as muscle, brain, spleen, lung, kidney, skin etc and has been shown to implicate in certain diseases including cardiac myopathies, neurodegenerative diseases and malignancies including brain, breast, prostate and renal cancers. Defectsive CRYAB causes myopathy myofibrillar type 2 (MFM2), cataract posterior polar type 2 (CTPP2) and myopathy myofibrillar fatal infantile hypertonic alpha-B crystallin-related (MFMFIH-CRYAB).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AlphaB Crystallin/CRYAB Antibody (NB100-2520)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ELISA, FACS.

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Product General Protocols

View specific protocols for AlphaB Crystallin/CRYAB Antibody (NB100-2520): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AlphaB Crystallin/CRYAB Antibody (NB100-2520). (Showing 1 - 1 of 1 FAQs).

  1. I expect it to be proprietary, but would it be possible to check if position 78 is definitely not part of the immunogen? I know that on the webpage the immunogen is mentioned to be aa 100-175.
    • The immunogen for NB100-2520 lies within aa 100-175. I can confirm that it does not include aa 78.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-2520AF405
Alexa Fluor (R) 488 Labeled NB100-2520AF488
Alexa Fluor (R) 647 Labeled NB100-2520AF647
Alexa Fluor (R) 700 Labeled NB100-2520AF700
Biotin Labeled NB100-2520B
DyLight 405 Labeled NB100-2520V
DyLight 488 Labeled NB100-2520G
DyLight 550 Labeled NB100-2520R
DyLight 650 Labeled NB100-2520C
FITC Labeled NB100-2520F
HRP Labeled NB100-2520H

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Contact Information

Product PDFs


Gene Symbol CRYAB

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