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Alpha Actinin 2 Antibody (JF0980)

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Western Blot: Alpha Actinin 2 Antibody (JF0980) [NBP2-67143] - Analysis of ACTN2 on human heart lysates using anti-ACTN2 antibody at 1/1,000 dilution.
Western Blot: Alpha Actinin 2 Antibody (JF0980) [NBP2-67143] - Analysis of ACTN2 on human heart lysates using anti-ACTN2 antibody at 1/1,000 dilution.
Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody (JF0980) [NBP2-67143] - Analysis of paraffin-embedded rat skeletal muscle tissue using anti-ACTN2 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody (JF0980) [NBP2-67143] - Analysis of paraffin-embedded mouse heart tissue using anti-ACTN2 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody (JF0980) [NBP2-67143] - Analysis of paraffin-embedded mouse skeletal tissue using anti-ACTN2 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody (JF0980) [NBP2-67143] - Analysis of paraffin-embedded rat heart tissue using anti-ACTN2 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clone
JF0980
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Alpha Actinin 2 Antibody (JF0980) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
recombinant protein (SwissProt: P35609 Human ;SwissProt: Q9JI91 Mouse ;EntrezGene: 291245 Rat)
Localization
Cytoplasm.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
ACTN2
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Alpha Actinin 2 Antibody (JF0980)

  • Actinin alpha 2
  • actinin, alpha 2
  • Actinin-a2
  • ACTN2
  • alpha Actinin 2
  • alpha-Actinin 2
  • Alpha-actinin skeletal muscle isoform 2
  • Alpha-Actinin Skeletal Muscle
  • Alpha-Actinin-2
  • CMD1AA
  • F-actin cross-linking protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 3 antibody diluted by 1:10000.
Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE; antibody diluted at 1:10000.
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Immunohistochemistry-Paraffin: Dystrophin Antibody [NBP1-89953] - Staining of human skeletal muscle shows strong membranous positivity in myocytes.
Immunohistochemistry-Paraffin: Dystrophin Antibody [NBP1-89953] - Staining of human tonsil shows no positivity as expected.
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Immunohistochemistry-Paraffin: Titin Antibody [NBP1-88071] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.
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Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - The blots show the examples of immunobands against NMDAR1 and beta-actin antibodies on the membrane, which was performed on the membrane protein prepared from the lumbar spinal cords of the offspring (P14) rats in different groups. Image collected and cropped by CiteAb from the following publication (http://www.jbiomedsci.com/content/18/1/64) licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - Mouse neuron stained with anti-NMDAR1 antibody. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - Mixed rat cortical cultures (21 DIV) were labelled with anti-NMDAR1 (1:200) and anti-mouse CF568 (1:250). The antibody labels small punctae along the neuronal processes. No distinct features such as spines are visible. This image was submitted via customer Review.
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Flow Cytometry: NMDAR2B Antibody [NB300-106] - NMDA2B receptor expression on CD4+ T cells. Image supplied by review from confirmed customer.
Western Blot: NMDAR2B Antibody [NB300-106]
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Immunohistochemistry: NDOR1 Antibody (OTI1E3) [NBP2-45856] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. C6 glioma cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
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Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983] - staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Western Blot: NMDAR2A Antibody [NB300-105] - Hyperpalmitoylation of Fyn kinase and GluN2B is reversed in Ppt1-/- primary cortical neurons by palmitoylation inhibitor treatment. Representative post-APEGS immunoblot of GluN2A (NMDAR2A) with beta-actin loading control. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/40316), licensed under a CC-BY licence.
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Western Blot: TCAP Antibody [NBP1-85544] - Analysis in human skeletal muscle tissue.
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Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) [NB100-74340] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Actin (mAbGEa) at 5 ug/mL overnight at 4C and detected with an anti-Mouse IgM DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Total protein from HeLa, 3T3, PC12 and Bovine normal tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Actin in 5% blocking buffer and detected with an anti-mouse IgM secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) [NB100-74340] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Actin Antibody (mAbGEa) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of Hela cells transiently transfected by pCMV6-ENTRY Alpha Actinin 1.
Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human bladder tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.
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I/CA1 Antibody
NBP1-88191
Western Blot: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Analysis in human cell line HELA.
Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Analysis in human rectum and pancreas tissues. Corresponding CA1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Staining of human duodenum shows no positivity in glandular cells as expected.
(3 Publications)
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Myotilin Antibody
NBP2-38361
Immunohistochemistry-Paraffin: Myotilin Antibody [NBP2-38361] - Staining of human skeletal muscle shows high expression.
Immunohistochemistry-Paraffin: Myotilin Antibody [NBP2-38361] - Staining of human skin shows low expression as expected.
Immunohistochemistry-Paraffin: Myotilin Antibody [NBP2-38361] - Staining in human skeletal muscle and skin tissues using anti-MYOT antibody. Corresponding MYOT RNA-seq data are presented for the same tissues.
Species: Hu
Applications: IHC, IHC-P
PDLIM5 Antibody
NBP1-84843
Immunocytochemistry/Immunofluorescence: PDLIM5 Antibody [NBP1-84843] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & focal adhesion sites.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining in human heart muscle and testis tissues.. Corresponding PDLIM5 RNA-seq data are presented for the same tissues.
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Alpha Actinin 2
Antibody (JF0980)
NBP2-67143
Alpha Actinin 2 Antibody (JF0980)
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P

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Bioinformatics

Gene Symbol ACTN2