alpha-2B Adrenergic R/ADRA2B Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

alpha-2B Adrenergic R/ADRA2B Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human alpha-2B Adrenergic R/ADRA2B.
  
  

  Source: E. coli

  Amino Acid Sequence: IAKRSNRRGPRAKGGPGQGESKQPRPDHGGALASAKLPALASVASAREVN

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: ADRA2B
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-49470.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for alpha-2B Adrenergic R/ADRA2B Recombinant Protein Antigen

• a-2B AdrenergicR
• ADRA2B adrenergic, alpha-2B-, receptor
• ADRA2B
• ADRA2L1
• ADRA2L1adrenergic receptor alpha 2B
• ADRA2RL1
• ADRARL1
• adrenergic, alpha-2B-, receptor
• Alpha-2 adrenergic receptor subtype C2
• alpha-2-adrenergic receptor-like 1
• alpha-2B Adrenergic R
• alpha-2B adrenergic receptor
• alpha2B AdrenergicR
• Alpha-2B adrenoceptor
• Alpha-2B adrenoreceptor
• alpha-2B-adrenergic receptor
• ALPHA2BAR
• alpha-2BAR
• G-protein coupled receptor

Background

Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. This gene encodes the alpha2B subtype, which was observed to associate with eIF-2B, a guanine nucleotide exchange protein that functions in regulation of translation. A polymorphic variant of the alpha2B subtype, which lacks 3 glutamic acids from a glutamic acid repeat element, was identified to have decreased G protein-coupled receptor kinase-mediated phosphorylation and desensitization; this polymorphic form is also associated with reduced basal metabolic rate in obese subjects and may therefore contribute to the pathogenesis of obesity. This gene contains no introns in either its coding or untranslated sequences. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ADRA2B