alpha-2B Adrenergic R/ADRA2B Antibody (491613) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) human ADRA2B or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human ADRA2B Monoclonal Antibody (Catalog # MAB10324) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 491613
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

alpha-2B Adrenergic R/ADRA2B Antibody (491613) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human alpha ‑2B Adrenergic R/ADRA2B
  Accession # P18089
• Specificity: Detects human alpha ‑2B Adrenergic R/ADRA2B in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-2B Adrenergic R/ADRA2B Antibody (491613) [Unconjugated]

• a-2B AdrenergicR
• ADRA2B adrenergic, alpha-2B-, receptor
• ADRA2B
• ADRA2L1
• ADRA2L1adrenergic receptor alpha 2B
• ADRA2RL1
• ADRARL1
• adrenergic, alpha-2B-, receptor
• Alpha-2 adrenergic receptor subtype C2
• alpha-2-adrenergic receptor-like 1
• alpha-2B Adrenergic R
• alpha-2B adrenergic receptor
• alpha2B AdrenergicR
• Alpha-2B adrenoceptor
• Alpha-2B adrenoreceptor
• alpha-2B-adrenergic receptor
• ALPHA2BAR
• alpha-2BAR
• G-protein coupled receptor

Background

Alpha-2-adrenergic receptors, including ADRA2B, are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C that have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. ADRA2B was observed to associate with eIF-2B, a guanine nucleotide exchange protein that functions in regulation of translation. A polymorphic variant of ADRA2B was identified to have decreased G protein-coupled receptor kinase-mediated phosphorylation and desensitization; this polymorphic form is also associated with reduced basal metabolic rate in obese subjects and may therefore contribute to the pathogenesis of obesity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P18089()