alpha-2A Adrenergic R/ADRA2A Antibody (1006614) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) human ADRA2A or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human ADRA2A Monoclonal Antibody (Catalog # MAB10129) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 1006614
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

alpha-2A Adrenergic R/ADRA2A Antibody (1006614) [Unconjugated] Summary

• Immunogen: Synthetic peptide containing Human alpha ‑2A Adrenergic R/ADRA2A
• Specificity: Detects human alpha ‑2A Adrenergic R/ADRA2A in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-2A Adrenergic R/ADRA2A Antibody (1006614) [Unconjugated]

• a-2A AdrenergicR
• ADRA2
• ADRA2A
• ADRA2Ralpha-2-adrenergic receptor, platelet type
• ADRARZNF32
• adrenergic, alpha-2A-, receptor
• Alpha-2 adrenergic receptor subtype C10
• alpha-2A Adrenergic R
• alpha-2A adrenergic receptor
• alpha2A AdrenergicR
• Alpha-2A adrenoceptor
• Alpha-2A adrenoreceptor
• alpha-2AAR subtype C10
• ALPHA2AAR
• Alpha-2AAR
• ZNF32

Background

Alpha-2-adrenergic receptors, including ADRA2A, are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both ADRA2A and ADRA2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons. The ADRA2A subtype inhibited transmitter release at high stimulation frequencies, whereas the ADRA2C subtype modulated neurotransmission at lower levels of nerve activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.