alpha-2A Adrenergic R/ADRA2A Antibody (1006614) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 1006614
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

alpha-2A Adrenergic R/ADRA2A Antibody (1006614) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: Synthetic peptide containing Human alpha ‑2A Adrenergic R/ADRA2A
• Specificity: Detects human alpha ‑2A Adrenergic R/ADRA2A in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for alpha-2A Adrenergic R/ADRA2A Antibody (1006614) [CoraFluor™ 1]

• a-2A AdrenergicR
• ADRA2
• ADRA2A
• ADRA2Ralpha-2-adrenergic receptor, platelet type
• ADRARZNF32
• adrenergic, alpha-2A-, receptor
• Alpha-2 adrenergic receptor subtype C10
• alpha-2A Adrenergic R
• alpha-2A adrenergic receptor
• alpha2A AdrenergicR
• Alpha-2A adrenoceptor
• Alpha-2A adrenoreceptor
• alpha-2AAR subtype C10
• ALPHA2AAR
• Alpha-2AAR
• ZNF32

Background

This study investigates the involvement of alpha2-adrenergic receptors (AR) in mouse brain induced by a low dose of methamphetamine (METH, 2 mg/kg). Immunohistochemical studies show that alpha2A-AR increased in the dentate gyrus area of the hippocampus 24 h after five repeated administrations of METH. The hippocampal alpha2A-AR proteins rose 3.2-fold when compared to the saline-administered mice. The other adrenergic receptor, alpha1D-AR, were not changed by the treatment. Moreover, alphao-subunits of GTP-binding proteins (Galphao), one of the downstream molecules of alpha2A-AR, was also increased by the treatment. These suggest that the repeated administration of low-doses of METH causes quantitative changes of the signaling of alpha2A-AR in the mouse hippocampus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.